Beuchat L R, Ward T E, Pettigrew C A
Center for Food Safety and Quality Enhancement, Department of Food Science and Technology, University of Georgia, Griffin 30223-1797, USA.
J Food Prot. 2001 Feb;64(2):152-8. doi: 10.4315/0362-028x-64.2.152.
Outbreaks of Salmonella and Escherichia coli O157:H7 infections associated with alfalfa and other seed sprouts have occurred with increased frequency in recent years. This study was undertaken to determine the efficacy of a liquid prototype produce wash product (Fit), compared with water and chlorinated water, in killing Salmonella and E. coli O157:H7 inoculated onto alfalfa seeds. We investigated the efficacy of treatments as influenced by seeds from two different lots obtained from two seeds suppliers and by two methods of inoculation. The efficacy of treatments was influenced by differences in seed lots and amount of organic material in the inoculum. Significant (alpha = 0.05) reductions in Salmonella populations on seeds treated with 20,000 ppm of chlorine or Fit for 30 min ranged from 2.3 to 2.5 log10 CFU/g and 1.7 to 2.3 log10 CFU/g, respectively. Reductions (alpha = 0.05) in E. coli O157:H7 ranged from 2.0 to 2.1 log10 CFU/g and 1.7 to more than 5.4 log10 CFU/g of seeds treated, respectively, with 20,000 ppm of chlorine or Fit. Compared with treatment with 200 ppm of chlorine, treatment with either 20,000 ppm of chlorine or Fit resulted in significantly higher reductions in populations of Salmonella and E. coli O157:H7. None of the treatments eliminated these pathogens as evidenced by their detection on enrichment of treated seeds. Considering the human health and environmental hazards associated with the use of 20,000 ppm of chlorine, Fit provides an effective alternative to chlorine as a treatment to significantly reduce bacterial pathogens that have been associated with alfalfa seeds.
近年来，与苜蓿及其他豆芽相关的沙门氏菌和大肠杆菌O157:H7感染暴发频率有所增加。本研究旨在确定一种液体原型农产品清洗产品（Fit）与水和加氯水相比，在杀灭接种于苜蓿种子上的沙门氏菌和大肠杆菌O157:H7方面的效果。我们研究了来自两家种子供应商的两个不同批次种子以及两种接种方法对处理效果的影响。处理效果受种子批次差异和接种物中有机物质含量的影响。用20000 ppm氯或Fit处理种子30分钟后，沙门氏菌数量显著（α = 0.05）减少，分别为2.3至2.5 log10 CFU/g和1.7至2.3 log10 CFU/g。用20000 ppm氯或Fit处理的种子，大肠杆菌O157:H7数量减少（α = 0.05）分别为2.0至2.1 log10 CFU/g和1.7至超过5.4 log10 CFU/g。与用200 ppm氯处理相比，用20000 ppm氯或Fit处理导致沙门氏菌和大肠杆菌O157:H7数量显著更多地减少。经处理种子富集检测发现，没有一种处理能消除这些病原体。考虑到使用20000 ppm氯对人类健康和环境的危害，Fit可作为氯的有效替代品，用于显著减少与苜蓿种子相关的细菌病原体。
