van Eekert M H, Schröder T J, van Rhee A, Stams A J, Schraa G, Field J A
Department of Biomolecular Sciences, Laboratory of Microbiology, Wageningen University, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands.
Bioresour Technol. 2001 Apr;77(2):163-70. doi: 10.1016/s0960-8524(00)00149-8.
The ability of granular methanogenic sludge to dechlorinate chloroethenes was investigated with unadapted sludge from an upflow anaerobic sludge blanket (UASB) reactor fed with methanol. The sludge degraded chlorinated ethenes, but the degradation rates were low. The addition of primary substrate was necessary to sustain dechlorination. The dechlorinating activity seemed to be constitutively present in the anaerobic bacteria. Usually, one chlorine atom was removed via reductive hydrogenolysis. Only trichloroethene (TCE) was converted to substantial amounts of vinylchloride (VC). 1,1-Dichloroethene (1,1DCE) was observed to be an important intermediate in the dechlorination by unadapted granular sludge, although previously this compound had not been commonly observed. Furthermore, the dechlorination of 1,1DCE was faster than the dechlorination of the other chloroethenes.
采用来自以甲醇为进料的上流式厌氧污泥床（UASB）反应器的未驯化污泥，研究了颗粒状产甲烷污泥对氯乙烯进行脱氯的能力。该污泥能降解氯代乙烯，但降解速率较低。需要添加主要底物来维持脱氯过程。脱氯活性似乎在厌氧细菌中是组成性存在的。通常，通过还原性氢解作用去除一个氯原子。只有三氯乙烯（TCE）能转化为大量的氯乙烯（VC）。观察到1,1 - 二氯乙烯（1,1DCE）是未驯化颗粒污泥脱氯过程中的重要中间产物，尽管此前该化合物并不常见。此外，1,1DCE的脱氯速度比其他氯代乙烯的脱氯速度快。
