Chen K T, Lin J D, Chao T C, Hsueh C, Chang C A, Weng H F, Chan E C
School of Medical Technology, Chang Gung University, Taiwan.
Thyroid. 2001 Jan;11(1):41-6. doi: 10.1089/10507250150500658.
Patients with follicular thyroid carcinoma have a higher incidence of metastasis than papillary thyroid carcinoma when thyroid cancer is diagnosed. The cDNA expression array technology is utilized herein to profile differentially expressed genes from metastatic human follicular thyroid carcinoma and reveal new tumor markers as well as target genes for therapeutic intervention. Tissue samples were obtained during surgical resection of the thyroid follicular carcinoma and metastatic tissue in the brain of the same patient. Two identical Atlas human cDNA expression arrays were hybridized with 32P-labeled cDNA probes derived from RNA of either primary thyroid cancer or metastatic tissue. Parallel analysis of the hybridized signals allowed us to identify the alteration of gene expression in the metastasis process. Eighteen genes significantly overexpressed and 40 genes significantly underexpressed were identified in the metastatic thyroid cancer. Genes that displayed an altered expression were associated with the processes of cell cycle regulation, apoptosis, DNA damage response, angiogenesis, cell adhesion and mobility, invasion, and immune response. An expression profile of genes that are associated with metastasis process of follicular thyroid cancer was also discussed. Further investigation is required to understand the precise relationship between the altered expression of these genes and the metastasis process of follicular thyroid cancer.
在甲状腺癌被诊断时，滤泡状甲状腺癌患者的转移发生率高于乳头状甲状腺癌。本文利用cDNA表达阵列技术分析转移性人类滤泡状甲状腺癌中差异表达的基因，以揭示新的肿瘤标志物以及治疗干预的靶基因。在对同一患者的甲状腺滤泡癌进行手术切除以及对其脑部转移组织进行手术时获取组织样本。将两个相同的阿特拉斯人类cDNA表达阵列与源自原发性甲状腺癌或转移组织RNA的32P标记的cDNA探针杂交。对杂交信号的平行分析使我们能够识别转移过程中基因表达的变化。在转移性甲状腺癌中鉴定出18个基因显著过表达，40个基因显著低表达。表达发生改变的基因与细胞周期调控、细胞凋亡、DNA损伤反应、血管生成、细胞黏附与迁移、侵袭及免疫反应等过程相关。还讨论了与滤泡状甲状腺癌转移过程相关的基因表达谱。需要进一步研究以了解这些基因表达改变与滤泡状甲状腺癌转移过程之间的确切关系。
