Vacca L L, Rosario S L, Zimmerman E A, Tomashefsky P, Po-Ying N G, Hsu K C
J Histochem Cytochem. 1975 Mar;23(3):208-15. doi: 10.1177/23.3.1127219.
Immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. Compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear more sensitive for the demonstration of intraneuronal horseradish peroxidase after retrograde transport. The immunoperoxidase bridge techniques from reaction products of high quality which can easily be seen in fine processes.
本文介绍了免疫过氧化物酶技术，该技术可用于在中枢神经系统石蜡包埋组织中定位辣根过氧化物酶示踪剂。与使用冰冻切片的组织化学方法相比，这些免疫技术允许使用储存的连续石蜡切片，并且在逆行运输后对神经元内辣根过氧化物酶的显示似乎更敏感。免疫过氧化物酶桥技术能产生高质量的反应产物，在细微结构中很容易看到。
