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光学显微镜免疫细胞化学染色的可靠性标准。

Criteria of reliability for light microscopic immunocytochemical staining.

作者信息

Petrusz P, Ordronneau P, Finley J C

出版信息

Histochem J. 1980 May;12(3):333-48. doi: 10.1007/BF01006954.

DOI:10.1007/BF01006954
PMID:7440247
Abstract

The following criteria of reliability are defined and discussed for immunocytochemical staining at the light microscopical level: efficiency, accuracy, precision, sensitivity, and specificity. Whenever practical, tests are suggested for obtaining information on the extent to which these criteria are fulfilled in a given system, and procedures are outlined for improving immunocytochemical staining in terms of these criteria. It is suggested that consideration of reliability criteria will help investigators in their choice of methodology, design of experimental strategy, and valid interpretation of the results.

摘要

本文定义并讨论了在光学显微镜水平进行免疫细胞化学染色时的以下可靠性标准

效率、准确性、精密度、灵敏度和特异性。只要可行,建议进行测试以获取有关给定系统中这些标准满足程度的信息,并概述了根据这些标准改进免疫细胞化学染色的程序。建议考虑可靠性标准将有助于研究人员选择方法、设计实验策略以及对结果进行有效解释。

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Criteria of reliability for light microscopic immunocytochemical staining.光学显微镜免疫细胞化学染色的可靠性标准。
Histochem J. 1980 May;12(3):333-48. doi: 10.1007/BF01006954.
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Immunocytochemical localization of LH, FSH and TSH in the fetal porcine pituitary.促黄体生成素、促卵泡生成素和促甲状腺激素在胎猪垂体中的免疫细胞化学定位。
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J Histochem Cytochem. 1966 Apr;14(4):291-302. doi: 10.1177/14.4.291.
9
A new fluorescent antibody method: mixed antiglobulin immunofluorescence or labelled antigen indirect immunofluorescence staining.一种新的荧光抗体方法:混合抗球蛋白免疫荧光或标记抗原间接免疫荧光染色。
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10
Mucosal and glandular distribution of immunoglobulin components. Immunohistochemistry with a cold ethanol-fixation technique.免疫球蛋白成分的黏膜和腺体分布。采用冷乙醇固定技术的免疫组织化学。
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