Durand V, Pers J O, Renaudineau Y, Saraux A, Youinou P, Jamin C
Laboratory of Immunology, Institut de Synergie des Sciences et de la Santé, Brest University Medical School, France.
J Leukoc Biol. 2001 Feb;69(2):233-40.
Anti-Fc gamma receptor IIIb (Fc gammaRIIIb) human autoantibodies (Ab) have been classified previously into three groups, based on the results of an indirect immunofluorescence (IIF) test and an enzyme-linked immunosorbent assay (ELISA): IIF+/ELISA+ (group A), IIF+/ELISA- (group B), and IIF-/ELISA+ (group C) sera. In this study, differential effects between IIF+ autoAb, recognizing cell-bound Fc gammaR, and those ELISA+, recognizing only cell-free Fc gammaR, were studied on polymorphonuclear neutrophils (PMN). Neither group A nor B autoAb was cytotoxic, although both prolonged the survival of PMN by delaying spontaneous apoptosis. By the same extent, the PMN-binding antisera stimulated the appearance of a CD11b(dim) population, following a 12-h incubation. This event was associated with a lowered expression of beta2 integrin molecules, resulting in altered PMN function. Treatment with groups A and B autoAb reduced adhesiveness and respiratory burst. This impairment of the responses was more pronounced when the cells originated from donors NA1+ NA1+ rather than donors NA2+ NA2+. From our observations, the influences of anti-Fc gammaRIIIb autoAb on PMN survival, as well as function and subsequent dysregulation of the inflammatory response, have proven somewhat dependent on their target antigens, as determined by IIF coupled with ELISA and Fc gammaRIIIb polymorphism.
抗Fcγ受体IIIb（FcγRIIIb）人自身抗体（Ab）先前已根据间接免疫荧光（IIF）试验和酶联免疫吸附测定（ELISA）的结果分为三组：IIF+/ELISA+（A组）、IIF+/ELISA-（B组）和IIF-/ELISA+（C组）血清。在本研究中，对多形核中性粒细胞（PMN）研究了识别细胞结合型FcγR的IIF+自身抗体和仅识别游离型FcγR的ELISA+自身抗体之间的差异效应。A组和B组自身抗体均无细胞毒性，尽管两者都通过延迟自发凋亡延长了PMN的存活时间。同样程度地，PMN结合抗血清在孵育12小时后刺激了CD11b（dim）群体的出现。这一事件与β2整合素分子表达降低有关，导致PMN功能改变。用A组和B组自身抗体处理可降低粘附性和呼吸爆发。当细胞来源于NA1+ NA1+供体而非NA2+ NA2+供体时，这种反应的损害更为明显。根据我们的观察，抗FcγRIIIb自身抗体对PMN存活、功能以及随后炎症反应失调的影响在一定程度上取决于其靶抗原，这是通过IIF与ELISA以及FcγRIIIb多态性确定的。
