一阶导数分光光度法、薄层色谱-光密度法和高效液相色谱法测定盐酸醋丁洛尔及其酸致降解产物共存时的盐酸醋丁洛尔含量。
First derivative spectrophotometric, TLC-densitometric, and HPLC determination of acebutolol HCL in presence of its acid-induced degradation product.
作者信息
El-Gindy A, Ashour A, Abel-Fattah L, Shabana M M
机构信息
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
出版信息
J Pharm Biomed Anal. 2001 Feb;24(4):527-34. doi: 10.1016/s0731-7085(00)00451-9.
Three methods are presented for the determination of acebutolol HCl in presence of its acid-induced degradation product. The first method was based on measurement of the first derivative amplitude of acebutolol HCl at 266.6 nm. The second method was based on separation of acebutolol HCl from its acid-induced degradation product followed by densitometric measurement of the spots at 230 nm. The separation was carried out on silica gel 60 F254, using ethanol-glacial acetic acid (4:1, v/v) as mobile phase. Second order polynomial equation was used for the regression line. The third method was based on high performance liquid chromatographic (HPLC) separation of acebutolol HCl from its acid-induced degradation product on a reversed phase, ODS column using a mobile phase of methanol-water (55:45, v/v) with UV detection at 240 nm. The first derivative spectrophotometric method was utilized to investigate the kinetics of the acid degradation process at different temperatures.
本文介绍了三种在盐酸醋丁洛尔的酸诱导降解产物存在下测定盐酸醋丁洛尔的方法。第一种方法基于在266.6nm处测量盐酸醋丁洛尔的一阶导数振幅。第二种方法是先将盐酸醋丁洛尔与其酸诱导降解产物分离，然后在230nm处对斑点进行光密度测定。分离在硅胶60 F254上进行，使用乙醇 - 冰醋酸（4:1，v/v）作为流动相。回归线采用二阶多项式方程。第三种方法基于在反相ODS柱上，用甲醇 - 水（55:45，v/v）作为流动相，在240nm处进行紫外检测，将盐酸醋丁洛尔与其酸诱导降解产物进行高效液相色谱（HPLC）分离。利用一阶导数分光光度法研究了不同温度下酸降解过程的动力学。
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