Xu M G, Crimeen B, Ludford-Menting M J, Gan X, Russell S M, Gu M
Centre for Micro-Photonics, School of Biophysical Sciences and Electrical Engineering, Swinburne University of Technology, Hawthorn, Victoria, Australia.
Scanning. 2001 Jan-Feb;23(1):9-13. doi: 10.1002/sca.4950230102.
Three-dimensional (3-D) imaging of fluorescence resonance energy transfer (FRET) in human cells under two-photon excitation was demonstrated in this study. A sample was prepared by expressing a donor and an acceptor in living cells and using an antibody to secure the proximity of contact between the donor and the acceptor. The quenching of fluorescence emission of a donor in the double-labelled cells indicates the presence of FRET that occurred in these living cells. Because of the quadratic relation of the excitation power, 3-D localisation of FRET becomes possible.
