Ashman Ross, Crimeen-Irwin Blessing, Ludford-Menting Mandy, Russell Sarah, Gu Min
Centre for Micro-Photonics, School of Biophysical Sciences and Electronic Engineering, Swinburne University of Technology, Hawthorn, Australia.
Scanning. 2003 May-Jun;25(3):116-20. doi: 10.1002/sca.4950250303.
We show two-photon spectra and lifetimes acquired using conventional confocal microscopes equipped with an ultra-short pulsed laser and a time-gated intensified charge coupled device. We report on the two-photon spectra and lifetimes of Alexa350, enhanced green fluorescent protein (EGFP), EGFP-CD46, and Cy3 labelled antibodies. Cellular and extracellular EGFP two-photon spectra and lifetimes are compared.
我们展示了使用配备超短脉冲激光器和时间门控增强型电荷耦合器件的传统共聚焦显微镜获取的双光子光谱和寿命。我们报告了Alexa350、增强型绿色荧光蛋白（EGFP）、EGFP-CD46和Cy3标记抗体的双光子光谱和寿命。比较了细胞内和细胞外EGFP的双光子光谱和寿命。
