Rhyan J C, Gidlewski T, Roffe T J, Aune K, Philo L M, Ewalt D R
U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, National Wildlife Research Center, Fort Collins, Colorado 80521, USA.
J Wildl Dis. 2001 Jan;37(1):101-9. doi: 10.7589/0090-3558-37.1.101.
Between February 1995 and June 1999, specimens from seven aborted bison (Bison bison) fetuses or stillborn calves and their placentas, two additional placentas, three dead neonates, one 2-wk-old calf, and 35 juvenile and adult female bison from Yellowstone National Park (USA) were submitted for bacteriologic and histopathologic examination. One adult animal with a retained placenta had recently aborted. Serum samples from the 35 juvenile and adult bison were tested for Brucella spp. antibodies. Twenty-six bison, including the cow with the retained placenta, were seropositive, one was suspect, and eight were seronegative. Brucella abortus biovar 1 was isolated from three aborted fetuses and associated placentas, an additional placenta, the 2-wk-old calf, and 11 of the seropositive female bison including the animal that had recently aborted. Brucella abortus biovar 2 was isolated from one additional seropositive adult female bison. Brucella abortus was recovered from numerous tissue sites from the aborted fetuses, placentas and 2-wk-old calf. In the juvenile and adult bison, the organism was more frequently isolated from supramammary (83%), retropharyngeal (67%), and iliac (58%) lymph nodes than from other tissues cultured. Cultures from the seronegative and suspect bison were negative for B. abortus. Lesions in the B. abortus-infected, aborted placentas and fetuses consisted of necropurulent placentitis and mild bronchointerstitial pneumonia. The infected 2-wk-old calf had bronchointerstitial pneumonia, focal splenic infarction, and purulent nephritis. The recently-aborting bison cow had purulent endometritis and necropurulent placentitis. Immunohistochemical staining of tissues from the culture-positive aborted fetuses, placentas, 2-wk-old calf, and recently-aborting cow disclosed large numbers of B. abortus in placental trophoblasts and exudate, and fetal and calf lung. A similar study with the same tissue collection and culture protocol was done using six seropositive cattle from a B. abortus-infected herd in July and August, 1997. Results of the bison and cattle studies were similar.
1995年2月至1999年6月期间，来自美国黄石国家公园的7例流产美洲野牛胎儿或死产小牛及其胎盘、另外2个胎盘、3例死亡新生儿、1头2周龄小牛以及35头幼年和成年雌性美洲野牛的样本被提交进行细菌学和组织病理学检查。1头成年动物胎盘滞留，近期流产。对35头幼年和成年美洲野牛的血清样本进行了布鲁氏菌属抗体检测。26头美洲野牛血清呈阳性，包括胎盘滞留的母牛，1头疑似阳性，8头血清阴性。从3例流产胎儿及相关胎盘、另外1个胎盘、2周龄小牛以及11头血清阳性的雌性美洲野牛（包括近期流产的那头动物）中分离出牛种布鲁氏菌生物变种1。从另外1头血清阳性的成年雌性美洲野牛中分离出牛种布鲁氏菌生物变种2。从流产胎儿、胎盘和2周龄小牛的多个组织部位中分离出牛种布鲁氏菌。在幼年和成年美洲野牛中，该菌在乳房上淋巴结（83%）、咽后淋巴结（67%）和髂淋巴结（58%）中的分离频率高于其他培养组织。血清阴性和疑似阳性美洲野牛的培养物未检测到牛种布鲁氏菌。感染牛种布鲁氏菌的流产胎盘和胎儿的病变包括坏死性脓性胎盘炎和轻度支气管间质性肺炎。受感染的2周龄小牛患有支气管间质性肺炎、局灶性脾梗死和脓性肾炎。近期流产的美洲野牛母牛患有脓性子宫内膜炎和坏死性脓性胎盘炎。对培养阳性的流产胎儿、胎盘、2周龄小牛和近期流产母牛的组织进行免疫组织化学染色，结果显示胎盘滋养层细胞、渗出物以及胎儿和小牛的肺中有大量牛种布鲁氏菌。1997年7月和8月，对来自一个感染牛种布鲁氏菌的牛群中的6头血清阳性牛进行了相同组织采集和培养方案的类似研究。美洲野牛和牛的研究结果相似。
