Kilbourn A M, Godfrey H P, Cook R A, Calle P P, Bosi E J, Bentley-Hibbert S I, Huygen K, Andau M, Ziccardi M, Karesh W B
Wildlife Health Sciences, Wildlife Conservation Society, Bronx, New York 10460, USA.
J Wildl Dis. 2001 Jan;37(1):65-71. doi: 10.7589/0090-3558-37.1.65.
Diagnosis of active mycobacterial disease in orangutans (Pongo pygmaeus) has been impeded by high levels of non-specific intradermal skin test reactivity to mycobacterial antigens. This may be due in part to cross reactivity between antigens, tuberculin concentrations used or other species-specific factors. Antigen 85 (Ag85) complex proteins are major secretory products of actively growing mycobacteria, and measurement of serum Ag85 could provide a method for determining active mycobacterial infections that was not dependent on host immunity. Serum Ag85 was measured by dot-immunobinding assay using monoclonal anti-Ag85, purified Ag85 standard and enhanced chemiluminescence technology in coded serum samples from 14 captive orangutans from a zoo in Colorado, 15 semi-captive orangutans in Malaysia, and 19 free-ranging wild orangutans in Malaysia. Orangutans from Colorado (USA) were culture negative for Mycobacterium tuberculosis and M. avium, although all had laboratory suspicion or evidence of mycobacterial infection; median serum Ag85 was 10 microU/ml (range, <0.25-630 microU/ml). Of the semi-captive orangutans, six were skin test reactive and two were culture positive for M. avium on necropsy. Median serum Ag85 for this group was 1,880 microU/ml (0.75-7,000 microU/ml), significantly higher than that of Colorado zoo or free-ranging Malaysian orangutans. Median serum Ag85 in the latter group was 125 microU/ml (range, 0.75-2,500 microU/ml). These data suggest that suggest that additional studies using more specific reagents and more samples from animals of known status are appropriate.
对猩猩（婆罗洲猩猩）活动性分枝杆菌病的诊断一直受到其对分枝杆菌抗原高水平非特异性皮内皮肤试验反应性的阻碍。这可能部分归因于抗原之间的交叉反应、所用结核菌素浓度或其他物种特异性因素。抗原85（Ag85）复合蛋白是活跃生长的分枝杆菌的主要分泌产物，血清Ag85的测定可为确定不依赖宿主免疫的活动性分枝杆菌感染提供一种方法。使用单克隆抗Ag85、纯化的Ag85标准品和增强化学发光技术，通过斑点免疫结合试验对来自科罗拉多州一家动物园的14只圈养猩猩、马来西亚的15只半圈养猩猩以及马来西亚的19只自由放养野生猩猩的编码血清样本中的血清Ag85进行了测定。来自美国科罗拉多州的猩猩结核分枝杆菌和鸟分枝杆菌培养均为阴性，尽管所有猩猩在实验室检查中都有分枝杆菌感染的怀疑或证据；血清Ag85中位数为10微单位/毫升（范围为<0.25 - 630微单位/毫升）。在半圈养猩猩中，有6只皮试呈反应性，2只在尸检时鸟分枝杆菌培养呈阳性。该组血清Ag85中位数为1880微单位/毫升（0.75 - 7000微单位/毫升），显著高于科罗拉多动物园或自由放养的马来西亚猩猩。后一组血清Ag85中位数为125微单位/毫升（范围为0.75 - 2500微单位/毫升）。这些数据表明，使用更特异的试剂并从已知状态的动物获取更多样本进行进一步研究是合适的。
