Gomis P, Kacem K, Sercombe C, Seylaz J, Sercombe R
Laboratoire de Recherches Cérébrovasculaires, CNRS UPR. 646, Faculté de Médecine Lariboisière-Saint Louis, Paris, France.
Histochem J. 2000 Nov;32(11):673-8. doi: 10.1023/a:1004115432660.
Our objective was to determine whether subarachnoid haemorrhage modifies cerebral artery smooth muscle cell phenotype and the contractile protein alpha-actin measured 7 days after haemorrhage. We used a rabbit subarachnoid haemorrhage model and immunofluorescence labelling of alpha-smooth muscle actin, vimentin and desmin. The paired comparison between the haemorrhage and sham rabbits was performed using confocal laser-scanning microscopy. We found in the haemorrhage group significantly less intense alpha-actin immunostaining (p = 0.036) and more intense vimentin immunostaining (p = 0.043) but no significant change in the intensity of desmin staining. Our results indicate an absolute decrease after subarachnoid haemorrhage in the amount of functional alpha-actin and in the light of the literature may suggest a certain degree of dedifferentiation of smooth muscle cells in the cerebral artery wall.
我们的目的是确定蛛网膜下腔出血是否会改变出血7天后脑动脉平滑肌细胞表型及收缩蛋白α-肌动蛋白。我们使用了兔蛛网膜下腔出血模型，并对α-平滑肌肌动蛋白、波形蛋白和结蛋白进行免疫荧光标记。采用共聚焦激光扫描显微镜对出血组和假手术组兔子进行配对比较。我们发现，出血组α-肌动蛋白免疫染色强度显著降低（p = 0.036），波形蛋白免疫染色强度增强（p = 0.043），但结蛋白染色强度无显著变化。我们的结果表明，蛛网膜下腔出血后功能性α-肌动蛋白的量绝对减少，根据文献，这可能提示脑动脉壁平滑肌细胞存在一定程度的去分化。
