支原体生长的微量热法检测

Microcalorimetric detection of growth of Mycoplasmatales.

作者信息

Ljungholm K, Wadsö I, Mårdh P A

出版信息

J Gen Microbiol. 1976 Oct;96(2):283-8. doi: 10.1099/00221287-96-2-283.

Abstract

A static ampoule microcalorimeter was used to study the growth of mycoplasmas, acholeplasmas and ureaplasmas. Growth as indicated by thermograms was compared with the results of conventional methods, namely, terminal dilution counts, plate counts, turbidimetric measurements, glucose consumption and pH changes. Removal of oxygen had little effect on mycoplasma growth. The microcalorimetric method is potentially useful for identifying and enumerating the members of the Mycoplasmatales.

摘要

使用静态安瓿微量量热计研究支原体、无胆甾原体和脲原体的生长情况。将热谱图所示的生长情况与传统方法的结果进行比较，传统方法包括终点稀释计数、平板计数、比浊法测量、葡萄糖消耗和pH变化。去除氧气对支原体生长影响不大。微量量热法在鉴定和计数支原体目成员方面可能具有实用价值。