硫酸黏菌素作为乙酸铊在用于支原体检测和培养的培养基中的合适替代品。

Colistin sulfate as a suitable substitute of thallium acetate in culture media intended for mycoplasma detection and culture.

作者信息

Angulo Alejandro F, Jacobs Marcel V, van Damme Ellen H A, Akkermans Arnoud M, de Kruijff-Kroesen Ingrid, Brugman Jan

机构信息

National Institute for Public Health and the Environment, P.O. Box 1, 3720 BA, Bilthoven, Netherlands.

出版信息

Biologicals. 2003 Sep;31(3):161-3. doi: 10.1016/s1045-1056(03)00031-9.

DOI:10.1016/s1045-1056(03)00031-9

PMID:12935803

Abstract

Thallium acetate in concentrations of 500 to 1000 mg/l is tolerated in the culture by the most mollicutes of the orders Mycoplasmatales and Acholeplasmatales and by this reason it is added in the culture media as a selective element for the detection and propagation of mycoplasmas and acholeplasmas. Because of the high toxicity of thallium acetate and its accumulation in the environment, thallium acetate is not biodegradable, an alternative was searched. The results and analysis of tests with nine mollicute species are presented here. It is recommended to replace thallium acetate in the formulations where it is used and colistin sulfate is proposed as its substitute.

摘要

醋酸铊浓度为500至1000毫克/升时，支原体目和无胆甾原体目的大多数柔膜菌能耐受，因此它被添加到培养基中作为检测和培养支原体及无胆甾原体的选择因子。由于醋酸铊毒性高且会在环境中蓄积，且不可生物降解，因此需要寻找替代物。本文给出了对9种柔膜菌进行试验的结果及分析。建议在使用醋酸铊的配方中用硫酸黏菌素替代它。