Bradwell A R, Carr-Smith H D, Mead G P, Tang L X, Showell P J, Drayson M T, Drew R
Department of Immunology, The Medical School, Edgbaston, Birmingham B15 2TT, United Kingdom.
Clin Chem. 2001 Apr;47(4):673-80.
Bence Jones proteins or monoclonal immunoglobulin kappa and lambda free light chains (FLCs) are important markers for identifying and monitoring many patients with B-cell tumors. Automated immunoassays that measure FLCs in urine and serum have considerable clinical potential.
Sheep antibodies, specific for FLCs, were prepared by immunization with pure kappa and lambda molecules and then adsorbed extensively against whole immunoglobulins. The antibodies were conjugated onto latex particles and used to assay kappa and lambda FLCs on the Beckman IMMAGE protein analyzer.
The unconjugated antibodies showed minimal cross-reactivity with intact immunoglobulins or other proteins. With latex-conjugated antibodies, kappa and lambda FLCs could be measured in normal sera and most normal urine samples. Patients with multiple myeloma had increased concentrations of the relevant serum FLC, whereas both FLCs were increased in the sera of patients with systemic lupus erythematosus.
We developed sensitive, automated immunoassays for kappa and lambda FLC measurements in serum and urine that should facilitate the assessment of patients with light chain abnormalities.
本周氏蛋白或单克隆免疫球蛋白κ和λ游离轻链（FLC）是识别和监测许多B细胞肿瘤患者的重要标志物。检测尿液和血清中FLC的自动化免疫测定具有相当大的临床潜力。
通过用纯κ和λ分子免疫制备对FLC特异的绵羊抗体，然后用全免疫球蛋白进行广泛吸附。将抗体偶联到乳胶颗粒上，并用于在贝克曼IMMAGE蛋白质分析仪上检测κ和λFLC。
未偶联的抗体与完整免疫球蛋白或其他蛋白质的交叉反应性最小。使用乳胶偶联抗体，可以在正常血清和大多数正常尿液样本中检测κ和λFLC。多发性骨髓瘤患者相关血清FLC浓度升高，而系统性红斑狼疮患者血清中两种FLC均升高。
我们开发了用于血清和尿液中κ和λFLC测量的灵敏、自动化免疫测定方法，这应有助于评估有轻链异常的患者。
