14个跨膜区段（TMS）的Tet(L)抗生素抗性蛋白的12个跨膜区段版本（DeltaTMS VII-VIII）保留单价阳离子转运模式，但缺乏四环素外排能力。
Twelve-transmembrane-segment (TMS) version (DeltaTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity.
作者信息
Jin J, Guffanti A A, Beck C, Krulwich T A
机构信息
Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029, USA.
出版信息
J Bacteriol. 2001 Apr;183(8):2667-71. doi: 10.1128/JB.183.8.2667-2671.2001.
Abstract
A "Tet(L)-12" version of Tet(L), a tetracycline efflux protein with 14 transmembrane segments (TMS), was constructed by deletion of two central TMS. Tet(L)-12 catalyzed Na+/H+ antiport and antiport with K+ as a coupling ion as well as or better than wild-type Tet(L) but exhibited no tetracycline-Me2+/H+ antiport in Escherichia coli vesicles.
摘要
Tet(L)是一种具有14个跨膜区段（TMS）的四环素外排蛋白，通过缺失两个中央TMS构建了一种“Tet(L)-12”版本的Tet(L)。Tet(L)-12催化Na⁺/H⁺反向转运以及以K⁺作为偶联离子的反向转运，其效果与野生型Tet(L)相当或更好，但在大肠杆菌囊泡中未表现出四环素-Me²⁺/H⁺反向转运。
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Twelve-transmembrane-segment (TMS) version (DeltaTMS VII-VIII) of the 14-TMS Tet(L) antibiotic resistance protein retains monovalent cation transport modes but lacks tetracycline efflux capacity.14个跨膜区段（TMS）的Tet(L)抗生素抗性蛋白的12个跨膜区段版本（DeltaTMS VII-VIII）保留单价阳离子转运模式，但缺乏四环素外排能力。
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J Mol Microbiol Biotechnol. 2001 Apr;3(2):237-46.
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The central cytoplasmic loop of the major facilitator superfamily of transport proteins governs efficient membrane insertion.转运蛋白主要易化子超家族的中央细胞质环决定了有效的膜插入。
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Two types of Bacillus subtilis tetA(L) deletion strains reveal the physiological importance of TetA(L) in K(+) acquisition as well as in Na(+), alkali, and tetracycline resistance.两种枯草芽孢杆菌tetA(L)缺失菌株揭示了TetA(L)在钾离子摄取以及钠、碱和四环素抗性方面的生理重要性。
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