对多功能四环素外排蛋白Tet(L)的选定基序、带电荷残基和半胱氨酸进行的定点诱变研究。
Site-directed mutagenesis studies of selected motif and charged residues and of cysteines of the multifunctional tetracycline efflux protein Tet(L).
作者信息
Jin Jie, Krulwich Terry A
机构信息
Department of Pharmacology and Biological Chemistry, Mount Sinai School of Medicine, New York, New York 10029, USA.
出版信息
J Bacteriol. 2002 Mar;184(6):1796-800. doi: 10.1128/JB.184.6.1796-1800.2002.
Abstract
All of the transmembrane glutamates of Tet(L) are essential for tetracycline (TET) resistance, and E397 has been shown to be essential for all catalytic modes, i.e., TET-Me(2+) and Na(+) efflux and K(+) uptake. Loop residues D74 and G70 are essential for TET flux but not for Na(+) or K(+) flux. A cysteineless Tet(L) protein exhibits all activities.
摘要
Tet(L) 的所有跨膜谷氨酸对于四环素（TET）抗性都是必不可少的，并且已证明E397对于所有催化模式都是必不可少的，即TET-Me(2+) 和Na(+) 外流以及K(+) 摄取。环残基D74和G70对于TET通量是必不可少的，但对于Na(+) 或K(+) 通量则不是。无半胱氨酸的Tet(L) 蛋白表现出所有活性。
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3
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J Biol Chem. 2000 Jul 28;275(30):22704-12. doi: 10.1074/jbc.m910354199.
5
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