Rudnick P A, Arcondéguy T, Kennedy C K, Kahn D
Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, INRA/CNRS, 31326 Castanet-Tolosan Cedex, France.
J Bacteriol. 2001 Apr;183(8):2682-5. doi: 10.1128/JB.183.8.2682-2685.2001.
To evaluate the role of uridylyl-transferase, the Sinorhizobium meliloti glnD gene was isolated by heterologous complementation in Azotobacter vinelandii. The glnD gene is cotranscribed with a gene homologous to Salmonella mviN. glnD1::Omega or mviN1::Omega mutants could not be isolated by a powerful sucrose counterselection procedure unless a complementing cosmid was provided, indicating that glnD and mviN are members of an indispensable operon in S. meliloti.
为了评估尿苷酰转移酶的作用,通过在棕色固氮菌中进行异源互补分离出了苜蓿中华根瘤菌的谷氨酰胺合成酶基因。谷氨酰胺合成酶基因与一个与沙门氏菌mviN同源的基因共转录。除非提供一个互补黏粒,否则无法通过强大的蔗糖反选择程序分离出谷氨酰胺合成酶基因1::Ω或mviN基因1::Ω突变体,这表明谷氨酰胺合成酶基因和mviN是苜蓿中华根瘤菌中一个不可或缺的操纵子的成员。