枯草芽孢杆菌染色体内新整合位点的开发及兼容表达盒的构建。
Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes.
作者信息
Härtl B, Wehrl W, Wiegert T, Homuth G, Schumann W
机构信息
Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany.
出版信息
J Bacteriol. 2001 Apr;183(8):2696-9. doi: 10.1128/JB.183.8.2696-2699.2001.
Abstract
The Bacillus subtilis lacA gene, coding for beta-galactosidase, has been explored as a new site able to accept DNA sequences from nonreplicating delivery vectors. Two such delivery expression vectors have been constructed and shown to be useful in obtaining regulated expression from the chromosomal location. In another experiment, it was shown that the integration of a regulatory gene at the lacA locus was able to control the expression of a transcriptional fusion at the amyE locus. These experiments demonstrate that both integration sites can be used simultaneously to obtain regulated expression of desired genes.
摘要
编码β-半乳糖苷酶的枯草芽孢杆菌lacA基因已被探索作为一个能够接受来自非复制型递送载体的DNA序列的新位点。已经构建了两种这样的递送表达载体，并证明它们可用于从染色体位置获得调控表达。在另一个实验中，表明在lacA位点整合一个调控基因能够控制amyE位点转录融合的表达。这些实验证明，这两个整合位点可同时用于获得所需基因的调控表达。
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本文引用的文献
1
A vector for systematic gene inactivation in Bacillus subtilis.一种用于枯草芽孢杆菌中系统基因失活的载体。
Microbiology (Reading). 1998 Nov;144 ( Pt 11):3097-3104. doi: 10.1099/00221287-144-11-3097.
2
The GroE chaperonin machine is a major modulator of the CIRCE heat shock regulon of Bacillus subtilis.GroE伴侣蛋白机器是枯草芽孢杆菌CIRCE热休克调节子的主要调节因子。
EMBO J. 1997 Aug 1;16(15):4579-90. doi: 10.1093/emboj/16.15.4579.
3
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.枯草芽孢杆菌中编码β-半乳糖苷酶的lacA基因及调控基因lacR的分离与鉴定。
J Bacteriol. 1997 Sep;179(17):5636-8. doi: 10.1128/jb.179.17.5636-5638.1997.
4
Integrative vectors for constructing single-copy transcriptional fusions between Bacillus subtilis promoters and various reporter genes encoding heat-stable enzymes.用于构建枯草芽孢杆菌启动子与编码热稳定酶的各种报告基因之间单拷贝转录融合体的整合载体。
Gene. 1996 Dec 5;182(1-2):33-6. doi: 10.1016/s0378-1119(96)00447-7.
5
A xylose-inducible Bacillus subtilis integration vector and its application.一种木糖诱导型枯草芽孢杆菌整合载体及其应用。
Gene. 1996 Nov 28;181(1-2):71-6. doi: 10.1016/s0378-1119(96)00466-0.
6
hrcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat shock genes.hrcA是枯草芽孢杆菌dnaK操纵子的第一个基因，编码I类热休克基因的负调控因子。
J Bacteriol. 1996 Feb;178(4):1088-93. doi: 10.1128/jb.178.4.1088-1093.1996.
7
Control of short leftward transcripts from the immunity and ori regions in induced coliphage lambda.诱导型大肠杆菌噬菌体λ中免疫区和ori区短向左转录本的调控
Mol Gen Genet. 1973 Nov 22;126(4):275-90. doi: 10.1007/BF00269438.
8
Structure of a beta-galactosidase gene of Bacillus stearothermophilus.嗜热脂肪芽孢杆菌β-半乳糖苷酶基因的结构
J Bacteriol. 1986 Jun;166(3):722-7. doi: 10.1128/jb.166.3.722-727.1986.
9
Construction of a single-copy integration vector and its use in analysis of regulation of the trp operon of Bacillus subtilis.单拷贝整合载体的构建及其在枯草芽孢杆菌色氨酸操纵子调控分析中的应用。
Gene. 1986;43(1-2):85-94. doi: 10.1016/0378-1119(86)90011-9.
10
The family of highly interrelated single-stranded deoxyribonucleic acid plasmids.高度相互关联的单链脱氧核糖核酸质粒家族。
Microbiol Rev. 1989 Jun;53(2):231-41. doi: 10.1128/mr.53.2.231-241.1989.
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