Daniel R A, Haiech J, Denizot F, Errington J
Sir William Dunn School of Pathology, University of Oxford, United Kingdom.
J Bacteriol. 1997 Sep;179(17):5636-8. doi: 10.1128/jb.179.17.5636-5638.1997.
We have isolated transposon insertions in the lacA gene encoding an endogenous beta-galactosidase of Bacillus subtilis. Upstream of the putative operon containing lacA is a negative regulator, lacR, which encodes a product related to a family of regulators that includes the lactose repressor, lacI, of Escherichia coli. New strains with insertions in the lacA gene should be of use in studies using lacZ fusions in B. subtilis.
我们已经分离出枯草芽孢杆菌内源性β-半乳糖苷酶编码基因lacA中的转座子插入片段。在含有lacA的假定操纵子上游是一个负调控因子lacR,它编码的产物与一类调控因子家族相关,该家族包括大肠杆菌的乳糖阻遏物lacI。在lacA基因中有插入片段的新菌株应可用于枯草芽孢杆菌中使用lacZ融合的研究。