Petzmann S, Ullmann R, Klemen H, Renner H, Popper H H
Institute of Pathology, Laboratory Environmental and Respiraory Pathology, University of Graz, Medical School, Austria.
Hum Pathol. 2001 Mar;32(3):333-8. doi: 10.1053/hupa.2001.22762.
Neuroendocrine lung tumors such as typical carcinoid, atypical carcinoid, small-cell lung carcinoma, and large-cell neuroendocrine carcinoma represent a variable group with different biologic characteristics and unclear genetical relationships. We investigated the pattern of allelic loss on chromosome arm 11q in 20 sporadic carcinoid tumors of the lung using 10 microsatellite markers. Loss of heterozygosity was found in 13 of 20 tumors. In 5 of 9 typical carcinoids, 3 distinct regions of allelic loss were identified: 11q13.1 (D11S1883), 11q14.3-11q21 (D11S906), and 11q25 (D11S910). Atypical carcinoids showed loss of heterozygosity at 4 different regions: the first, most proximal region at 11q13 between markers PYGM and D11S937; the second at 11q14.3-11q21 (D11S906); and the third and fourth defined by markers D11S939 (11q23.2-23.3) and D11S910 (11q25). However, the region 11q13 harboring the MEN1 gene was more frequently affected in atypical carcinoids (7 of 11) than in typical carcinoids (2 of 9). The high rate of allelic losses within chromosomal region 11q13 in atypical carcinoids emphasizes the importance of this region for tumor development. We also recognized that more aggressive atypical carcinoids defined by high mitotic counts, vascular invasion, and/or organ metastasis are combined with increased allelic losses. HUM PATHOL 32:333-338.
神经内分泌性肺肿瘤，如典型类癌、非典型类癌、小细胞肺癌和大细胞神经内分泌癌，是一组具有不同生物学特性且遗传关系不明的肿瘤。我们使用10个微卫星标记物，对20例散发性肺类癌肿瘤中11号染色体长臂上等位基因缺失模式进行了研究。20例肿瘤中有13例发现杂合性缺失。在9例典型类癌中的5例中，鉴定出3个不同的等位基因缺失区域：11q13.1（D11S1883）、11q14.3 - 11q21（D11S906）和11q25（D11S910）。非典型类癌在4个不同区域显示杂合性缺失：第一个也是最靠近近端的区域在11q13，位于标记物PYGM和D11S937之间；第二个在11q14.3 - 11q21（D11S906）；第三个和第四个由标记物D11S939（11q23.2 - 23.3）和D11S910（11q25）界定。然而，携带MEN1基因的11q13区域在非典型类癌（11例中的7例）中比在典型类癌（9例中的2例）中更频繁地受到影响。非典型类癌中11q13染色体区域内高频率的等位基因缺失强调了该区域对肿瘤发生发展的重要性。我们还认识到，由高有丝分裂计数、血管侵袭和/或器官转移所定义的侵袭性更强的非典型类癌与更多的等位基因缺失相关。《人类病理学》32:333 - 338。
