慢性肌醇可增加大鼠脑磷脂酰乙醇胺缩醛磷脂。

Chronic myo-inositol increases rat brain phosphatidylethanolamine plasmalogen.

作者信息

Pettegrew J W, Panchalingam K, Levine J, McClure R J, Gershon S, Yao J K

机构信息

Neurophysics Laboratory, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.

出版信息

Biol Psychiatry. 2001 Mar 1;49(5):444-53. doi: 10.1016/s0006-3223(00)00953-7.

DOI:10.1016/s0006-3223(00)00953-7

PMID:11274656

Abstract

BACKGROUND

Oral myo-inositol (12--18 g/day) has shown beneficial effect in placebo-controlled studies of major depression, panic disorder, and obsessive compulsive disorder, and preliminary data suggest it also may be effective in bipolar depression. Evidence linking antidepressant activity to membrane phospholipid alterations suggested the examination of acute and chronic myo-inositol effects on rat brain membrane phospholipid metabolism.

METHODS

With both (31)P nuclear magnetic resonance (NMR) and quantitative high-performance thin-layer chromatography (HPTLC; hydrolysis) methods, rat brain phospholipid levels were measured after acute (n = 20, each group) and chronic myo-inositol administration (n = 10, each group). With (31)P NMR, we measured myo-inositol rat brain levels after acute and chronic myo-inositol administration.

RESULTS

Brain myo-inositol increased by 17% after acute myo-inositol administration and by 5% after chronic administration, as compared with the control groups. Chronic myo-inositol administration increased brain phosphatidylethanolamine (PtdEtn) plasmalogen by 10% and decreased brain PtdEtn by 5%, thus increasing the ratio PtdEtn plasmalogen (PtdEtn-Plas)/PtdEtn by 15%. Phosphatidylethanolamine plasmalogen levels quantified by (31)P NMR and HPTLC were highly correlated. The validity and reliability of the (31)P NMR method for phospholipid analysis were demonstrated with phospholipid standards.

CONCLUSIONS

The observed alteration in the PtdEtn-Plas/PtdEtn ratio could provide insights into the therapeutic effect of myo-inositol in affective disorders.

摘要

背景

口服肌醇（12 - 18克/天）在重度抑郁症、恐慌症和强迫症的安慰剂对照研究中显示出有益效果，初步数据表明其在双相抑郁症中可能也有效。将抗抑郁活性与膜磷脂改变联系起来的证据提示了对大鼠脑膜磷脂代谢的急性和慢性肌醇效应进行研究。

方法

采用磷-31核磁共振（NMR）和定量高效薄层色谱法（HPTLC；水解法），在急性（每组n = 20）和慢性给予肌醇后（每组n = 10）测量大鼠脑磷脂水平。采用磷-31核磁共振，我们在急性和慢性给予肌醇后测量大鼠脑内肌醇水平。

结果

与对照组相比，急性给予肌醇后脑内肌醇增加了17%，慢性给予后增加了5%。慢性给予肌醇使脑磷脂酰乙醇胺（PtdEtn）缩醛磷脂增加了10%，使脑PtdEtn减少了5%，从而使PtdEtn缩醛磷脂（PtdEtn - Plas）/PtdEtn的比值增加了15%。通过磷-31核磁共振和HPTLC定量的磷脂酰乙醇胺缩醛磷脂水平高度相关。用磷脂标准品证明了磷-31核磁共振法用于磷脂分析的有效性和可靠性。