Hoffman-Kuczynski Beth, Reo Nicholas V
Department of Biochemistry and Molecular Biology, Wright State University School of Medicine, WSU Magnetic Resonance Laboratory, Cox Institute, Dayton, Ohio 45429, USA.
Neurochem Res. 2004 Apr;29(4):843-55. doi: 10.1023/b:nere.0000018859.23881.f9.
Plasmalogens are ether-linked phospholipids that are abundant in nervous tissues. Their biological role is unclear, but may involve membrane structure/function and antioxidant activities. This study further investigates a recent report that chronic administration of myo-inositol in rats increased brain phosphatidylethanolamine plasmalogen (PlsEtn). We examined the effects of myo-inositol administration on the incorporation of [2-(13)C]ethanolamine ([2-(13)C]Etn) into rat brain phospholipids using NMR spectroscopy. Rats received either acute myo-inositol (single dose) +/- [2-(13)C]Etn, or chronic myo-inositol (10-day treatment) + [2-(13)C]Etn. Controls received saline rather than myo-inositol. Acute myo-inositol produced a 68% increase in brain [myo-inositol] and an increase in the incorporation of [2-(13)C]Etn into phospholipids (P < .05). The PlsEtn/phosphatidylethanolamine ratio and the [PlsEtn] were increased by 27% and 30%, respectively. The PlsEtn content as a mole percentage of total phospholipids was elevated (P < or = .05). Acute administration of myo-inositol + ethanolamine illustrates a positive correlation between the brain [myo-inositol] and the biosynthesis of ethanolamine phospholipids, with preferential synthesis of PlsEtn.
缩醛磷脂是神经组织中含量丰富的醚键连接的磷脂。它们的生物学作用尚不清楚，但可能涉及膜结构/功能和抗氧化活性。本研究进一步调查了最近的一份报告，该报告称在大鼠中长期给予肌醇可增加脑磷脂酰乙醇胺缩醛磷脂（PlsEtn）。我们使用核磁共振波谱法研究了给予肌醇对[2-(13)C]乙醇胺（[2-(13)C]Etn）掺入大鼠脑磷脂的影响。大鼠接受急性肌醇（单次剂量）+/-[2-(13)C]Etn，或慢性肌醇（10天治疗）+[2-(13)C]Etn。对照组接受生理盐水而非肌醇。急性给予肌醇使脑中[肌醇]增加68%，并使[2-(13)C]Etn掺入磷脂增加（P<.05）。PlsEtn/磷脂酰乙醇胺比值和[PlsEtn]分别增加了27%和30%。PlsEtn含量占总磷脂的摩尔百分比升高（P≤.05）。急性给予肌醇+乙醇胺表明脑中[肌醇]与乙醇胺磷脂的生物合成之间呈正相关，且优先合成PlsEtn。
