Sanchez T, Moreno J J
Department of Physiology, Faculty of Pharmacy, Barcelona University, E-08028, Barcelona, Spain.
Biochem Pharmacol. 2001 Apr 1;61(7):811-6. doi: 10.1016/s0006-2952(01)00555-x.
Recently, we suggested that arachidonic acid and/or its cyclooxygenase pathway metabolites may be involved in regulating 3T6 fibroblast proliferation. In the present study we evaluate the role of high-molecular phospholipase A2 (PLA2) enzymes in the 3T6 fibroblast growth. Our results demonstrate that the cytosolic PLA2 inhibitor, arachidonyl trifluoromethylketone and the cytosolic calcium-independent PLA2 (iPLA2) inhibitor, bromoenol lactone, decrease arachidonic acid release and prostaglandin E2 production in 3T6 fibroblast cultures stimulated by fetal calf serum. These effects were correlated with the impairment of 3T6 fibroblast proliferation and DNA synthesis at the S/G2 boundary, which prolongs the S phase. These data suggest a role of iPLA2 in the control of 3T6 fibroblast growth.
最近，我们提出花生四烯酸及其环氧化酶途径代谢产物可能参与调节3T6成纤维细胞的增殖。在本研究中，我们评估了高分子量磷脂酶A2（PLA2）酶在3T6成纤维细胞生长中的作用。我们的结果表明，胞质型PLA2抑制剂花生四烯酰三氟甲基酮和胞质型钙非依赖性PLA2（iPLA2）抑制剂溴代烯醇内酯，可减少胎牛血清刺激的3T6成纤维细胞培养物中花生四烯酸的释放和前列腺素E2的产生。这些作用与3T6成纤维细胞在S/G2边界处的增殖和DNA合成受损相关，这延长了S期。这些数据表明iPLA2在控制3T6成纤维细胞生长中发挥作用。
