Willis C M, Britton L E, Reiche L, Wilkinson J D
Department of Dermatology, Amersham Hospital, Whielden Street, Amersham, Bucks, HP7 0JD, UK.
Eur J Dermatol. 2001 Mar-Apr;11(2):99-104.
There is increasing evidence that oxidative stress plays a role in the pathogenesis of acute irritant contact dermatitis. As part of on-going studies into the effect of irritant chemicals on the anti-oxidant enzyme systems in the skin, we have examined the changing levels of two classes of glutathione S-transferase in patch test reactions to dithranol and sodium lauryl sulphate, using quantitative immunocytochemistry. Although no changes were evident after 6 hrs, significant reductions in the density of staining for glutathione S-transferase alpha were seen with both irritants after 48 hrs and 96 hrs. Glutathione S-transferase pi levels were reduced to a lesser degree, reaching significance for dithranol at the 96 hrs time point only, and for sodium lauryl sulphate at 48 hrs only. The results support the hypothesis that oxidative stress plays a role in chemically-induced inflammation, not only in the case of irritants such as dithranol which are known to directly generate reactive oxygen species, but also with chemicals not generally associated with free radical generation.
越来越多的证据表明，氧化应激在急性刺激性接触性皮炎的发病机制中起作用。作为对刺激性化学物质对皮肤抗氧化酶系统影响的正在进行的研究的一部分，我们使用定量免疫细胞化学方法，检测了在对蒽林和十二烷基硫酸钠的斑贴试验反应中两类谷胱甘肽S-转移酶水平的变化。虽然6小时后没有明显变化，但48小时和96小时后，两种刺激物处理后谷胱甘肽S-转移酶α的染色密度均显著降低。谷胱甘肽S-转移酶π水平的降低程度较小，仅在96小时时间点时蒽林处理组达到显著水平，十二烷基硫酸钠处理组仅在48小时时达到显著水平。这些结果支持了氧化应激在化学诱导的炎症中起作用这一假说，不仅在已知直接产生活性氧的刺激物如蒽林的情况下如此，而且在通常与自由基产生无关的化学物质的情况下也是如此。
