Wilkens L, Bredt M, Flemming P, Becker T, Klempnauer J, Kreipe H H
Institute of Pathology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.
J Pathol. 2001 Apr;193(4):476-82. doi: 10.1002/path.825.
Liver cell adenomas (LCAs) are rare tumours which may be difficult to differentiate from low-grade hepatocellular carcinomas (HCCs). This study used comparative genomic hybridization (CGH) to look for cytogenetic aberrations which would serve to distinguish between these tumours. For this purpose, ten LCAs and six well-differentiated HCCs were analysed and the results were compared with those reported previously for 15 well-differentiated HCCs. Aberrations were seen in 2/10 LCAs: a gain of chromosome 7p was observed in one and gains of 17q and 20 in a second case. In 6/6 well-differentiated HCCs, up to 13 aberrations were detectable, with a mean of 7.2 aberrations per case in chromosome sites 1q, 4p, 4q, 5p, 5q, 6p, 6q, 7p, 7q, 8p, 8q, 10q, 11p, 13q, 14q, 16p, 16q, 17p, 17q, 20p, 20q, and 21q. Aberrations focused on gains or losses of six chromosome sites, 1q, 4q, 8p, 8q, 16p, and 17p; in all HCC samples, at least two of these sites were affected. None of these aberrations occurred in any of the LCAs analysed. CGH is therefore helpful in distinguishing between LCA and well-differentiated HCC. Detection of one or more of the six most frequent aberrations in HCC supports the diagnosis of carcinoma and makes LCA unlikely.
肝细胞腺瘤（LCA）是一种罕见肿瘤，可能难以与低级别肝细胞癌（HCC）相鉴别。本研究采用比较基因组杂交（CGH）技术寻找有助于区分这些肿瘤的细胞遗传学异常。为此，分析了10例LCA和6例高分化HCC，并将结果与之前报道的15例高分化HCC的结果进行比较。在2/10的LCA中发现了异常：1例观察到7号染色体短臂增益，另1例观察到17号染色体长臂和20号染色体增益。在6/6的高分化HCC中，可检测到多达13处异常，每个病例在1号染色体长臂、4号染色体短臂、4号染色体长臂、5号染色体短臂、5号染色体长臂、6号染色体短臂、6号染色体长臂、7号染色体短臂、7号染色体长臂、8号染色体短臂、8号染色体长臂、10号染色体长臂、11号染色体短臂、13号染色体长臂、14号染色体长臂、16号染色体短臂、16号染色体长臂、17号染色体短臂、17号染色体长臂、20号染色体短臂、20号染色体长臂和21号染色体长臂位点的平均异常数为7.2处。异常集中在6个染色体位点的增益或缺失，即1号染色体长臂、4号染色体长臂、8号染色体短臂、8号染色体长臂、16号染色体短臂和17号染色体短臂；在所有HCC样本中，至少有两个这些位点受到影响。在所分析的任何LCA中均未出现这些异常。因此，CGH有助于区分LCA和高分化HCC。检测到HCC中6种最常见异常中的一种或多种支持癌的诊断，并使LCA的可能性不大。
