Akin F J, Chamberlain W J, Chortyk O T
J Natl Cancer Inst. 1975 Apr;54(4):907-12.
Ten fractions separated from the neutral portion of cigarette smoke condensate (CSC) were tested on mouse skin for tumor-initiating activity and for their capacity to induce the enzyme aryl hydrocarbon hydroxylase (AHH). Tumor-initiating activity was confined primarily to the fraction containing more than 90% of the polynuclear aromatic hydrocarbons (PAH) in CSC. One other PAH-containing fraction was active. The combined initiating effect of these fractions was comparable to that of a 40-ppm solution of benzo[a]pyrene (BP), which is about 40 times the BP content of CSC. Some of the neutral fractions that have been demonstrated to cause tumor promotion in mice pretreated with 7,12-dimethylbenz-[aA1ANTHRACENE sere inactive as tumor initiators. The fractions that contained aromatic hydrocarbons induced mouse skin AHH levels twofold to sixfold after a single topical application. AHH-inducing activity was not, however, a reliable indicator of the carcinogenic potential of a fraction.
从香烟烟雾冷凝物（CSC）的中性部分分离出的十个馏分，在小鼠皮肤上测试其肿瘤启动活性以及诱导芳烃羟化酶（AHH）的能力。肿瘤启动活性主要局限于含有CSC中90%以上多核芳烃（PAH）的馏分。另一个含PAH的馏分也具有活性。这些馏分的联合启动效应与40 ppm苯并[a]芘（BP）溶液的效应相当，这大约是CSC中BP含量的40倍。一些已被证明能在经7,12 - 二甲基苯并[a]蒽预处理的小鼠中引起肿瘤促进作用的中性馏分，作为肿瘤启动剂是无活性的。单次局部应用后，含有芳烃的馏分可使小鼠皮肤AHH水平提高两倍至六倍。然而，AHH诱导活性并不是一个馏分致癌潜力的可靠指标。
