用于分析革兰氏阴性菌基因表达和基因功能的稳定的源自RK2的克隆载体。

Stable RK2-derived cloning vectors for the analysis of gene expression and gene function in gram-negative bacteria.

作者信息

Dombrecht B, Vanderleyden J, Michiels J

机构信息

F.A. Janssens Laboratory of Genetics, Katholieke Universiteit Leuven, Heverlee, Belgium.

出版信息

Mol Plant Microbe Interact. 2001 Mar;14(3):426-30. doi: 10.1094/MPMI.2001.14.3.426.

DOI:10.1094/MPMI.2001.14.3.426

PMID:11277442

Abstract

The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoterless gusA gene are suitable for the study of gene function or regulation in bacteria.

摘要

本文报道了几种稳定的源自RK2的克隆载体的构建，用于分析革兰氏阴性菌中的基因表达和功能。质粒稳定性由RK2 par位点或通过插入来自根瘤菌属NGR234的pNGR234a的spsAB或spsCD共生质粒稳定性位点赋予。这些载体带有多个克隆位点，可防止载体序列的通读转录活性。带有组成型nptII启动子或无启动子gusA基因的载体衍生物适用于研究细菌中的基因功能或调控。