Tie-Jun S S, Xu Z, Hökfelt T
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Neuroreport. 2001 Mar 26;12(4):739-43. doi: 10.1097/00001756-200103260-00025.
Using immunohistochemistry and in situ hybridization the expression of calcitonin gene-related peptide (CGRP) was studied in the mouse spinal cord under normal conditions and after unilateral rhizotomy and after local colchicine treatment. Under normal conditions a dense plexus of CGRP-immunoreactive (IR) fibres was observed in the superfical layers of dorsal horns with lower numbers of fibers in deeper laminae. Seven days after unilateral rhizotomy, there was a marked reduction of CGRP-IR fibres in the ipsilateral superfical layers and distinctly CGRP-IR neurons could be detected in the ipsilateral lamina III. CGRP mRNA-positive neurons were observed in lamina III in both the ipsilateral and contralateral dorsal horn. Colchicine treatment did not markedly increase the number of CGRP-IR neurons. The results suggest that CGRP is synthesized in local dorsal horn neurons of the mouse, and these neurons presumably participate in sensory processing.
利用免疫组织化学和原位杂交技术，研究了正常条件下、单侧神经根切断术后以及局部秋水仙碱处理后小鼠脊髓中降钙素基因相关肽（CGRP）的表达情况。正常条件下，在背角浅层观察到密集的CGRP免疫反应性（IR）纤维丛，深层纤维数量较少。单侧神经根切断术后7天，同侧浅层的CGRP-IR纤维明显减少，在同侧III层可检测到明显的CGRP-IR神经元。在同侧和对侧背角的III层均观察到CGRP mRNA阳性神经元。秋水仙碱处理并未显著增加CGRP-IR神经元的数量。结果表明，CGRP在小鼠局部背角神经元中合成，这些神经元可能参与感觉处理。
