Goto S G, Kimura M T
Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, 060-0810, Japan.
Mol Phylogenet Evol. 2001 Mar;18(3):404-22. doi: 10.1006/mpev.2000.0893.
Phylogenetic utility of the mitochondrial COI (cytochrome oxidase subunit I) and nuclear Gpdh (glycerol-3-phosphate dehydrogenase) genes was studied in the Drosophila melanogaster species group. The rate of substitution was higher in the COI gene than in the Gpdh gene. In addition, multiple substitutions, not only for transitional but also for transversional substitutions, occurred faster in the COI gene. None of the trees obtained using the COI gene supported the well-established monophyly of the ananassae subgroup. In addition, the incongruence length difference test, Templeton test, and partitioned Bremer support revealed that the trees based on the COI data are considerably different from those based on the Gpdh and the combined data set. Thus, the COI gene did not show good phylogenetic performance in the melanogaster group. The present analyses based on the Gpdh gene and the combined data set revealed that the ananassae subgroup branched off first in the melanogaster group followed by the montium subgroup and further by the melanogaster subgroup in contrast to the most recent phylogenetic hypothesis based on Amy multigenes.
在黑腹果蝇物种组中研究了线粒体COI（细胞色素氧化酶亚基I）和核Gpdh（甘油-3-磷酸脱氢酶）基因的系统发育效用。COI基因的替换率高于Gpdh基因。此外，不仅转换替换，而且颠换替换，在COI基因中发生得更快。使用COI基因得到的树没有一个支持已确立的阿纳萨种亚组的单系性。此外，不一致长度差异检验、Templeton检验和分区布勒默支持表明，基于COI数据的树与基于Gpdh和组合数据集的树有很大不同。因此，COI基因在黑腹果蝇组中没有表现出良好的系统发育性能。基于Gpdh基因和组合数据集的当前分析表明，与基于Amy多基因的最新系统发育假设相反，阿纳萨种亚组在黑腹果蝇组中首先分支，其次是蒙蒂种亚组，然后是黑腹果蝇亚组。
