Goff B A, Paley P J, Greer B E, Gown A M
Department of Obstetrics and Gynecology, University of Washington School of Medicine, Seattle, Washington 98195-6460, USA.
Gynecol Oncol. 2001 Apr;81(1):18-24. doi: 10.1006/gyno.2000.6105.
Preliminary studies have suggested that lung resistance protein (LRP), multidrug resistance protein (MRP), and p27 may be useful markers of chemoresistance. Our goal was to evaluate the expression of LRP, MRP, and p27 in normal ovaries and chemosensitive and chemoresistant ovarian carcinomas.
Fourteen women with normal ovaries and fifty women with epithelial ovarian carcinoma who underwent cytoreductive surgery from 1996 through 1998 had specimens stained with immunocytochemistry for LRP, MRP, and p27. All women received paclitaxel/platinum-based chemotherapy. Twenty-nine women had a disease-free survival (DFS) of at least 12 months after completion of chemotherapy (sensitive) and 21 women had persistent disease during treatment (resistant).
Evaluation of LRP expression revealed significant differences between the normal ovaries and cancers in both the epithelial (57% vs 90%, P = 0.03) and stromal (86% vs 32%, P = 0.001) components. Evaluation of MRP expression revealed significant differences between normal ovaries and cancers in the epithelial component (7% vs 66%, P = 0.001) but not in the stromal component (14% vs 4%, P = 0.1). Evaluation of p27 revealed significant reductions in expression in cancers compared with normal ovaries for both the epithelial (90% vs 55%, P = 0.02) and stromal (88% vs 5%, P = 0.001) components. Comparison between the chemosensitive and chemoresistant groups revealed no significant differences in expression of LRP and MRP, in either the epithelial or stromal component, but significantly lower levels of p27 were expressed in the epithelial component of the chemoresistant group (P = 0.01).
The expression of LRP, MRP, and p27 is significantly different in ovarian cancers compared with normal ovaries. Low levels of p27 expression are associated with chemoresistance; however, LRP and MRP expression are not prognostic for chemosensitivity.
初步研究表明，肺耐药蛋白（LRP）、多药耐药蛋白（MRP）和p27可能是化疗耐药的有用标志物。我们的目标是评估LRP、MRP和p27在正常卵巢组织以及化疗敏感和耐药的卵巢癌组织中的表达情况。
对1996年至1998年间接受细胞减灭术的14例正常卵巢组织标本和50例上皮性卵巢癌组织标本进行免疫细胞化学染色，检测LRP、MRP和p27的表达。所有患者均接受了紫杉醇/铂类化疗。29例患者化疗结束后无病生存期（DFS）至少为12个月（敏感组），21例患者在治疗期间疾病持续存在（耐药组）。
LRP表达评估显示，正常卵巢组织与癌组织在上皮成分（57%对90%，P = 0.03）和间质成分（86%对32%，P = 0.001）方面均存在显著差异。MRP表达评估显示，正常卵巢组织与癌组织在上皮成分（7%对66%，P = 0.001）方面存在显著差异，但间质成分方面无显著差异（14%对4%，P = 0.1）。p27评估显示，与正常卵巢组织相比，癌组织在上皮成分（90%对55%，P = 0.02）和间质成分（88%对5%，P = 0.001）中的表达均显著降低。化疗敏感组和耐药组之间比较显示，LRP和MRP在上皮或间质成分中的表达均无显著差异，但耐药组上皮成分中p27的表达水平显著较低（P = 0.01）。
与正常卵巢组织相比，卵巢癌组织中LRP、MRP和p27的表达存在显著差异。p27低表达与化疗耐药相关；然而，LRP和MRP的表达对化疗敏感性无预后价值。
