Re D, Benenson E, Beyer M, Gresch O, Draube A, Diehl V, Wolf J
University of Cologne, Department of Internal Medicine I, Joseph-Stelzmannstr. 9, 50924 Cologne, Germany.
Am J Hematol. 2001 May;67(1):6-9. doi: 10.1002/ajh.1068.
The mechanism of multinucleated cell formation in Hodgkin's disease has not yet been elucidated. We asked whether the giant multinucleated cells of the H-RS cell line L1236 develop via fusion of the predominant smaller cells. As a positive control for the fusion assay, human B cells from the B-cell lymphoma cell line BJA-B were split into two fractions, stained with the fluorochromes CMTMR and CMFDA, respectively, and fused using the polyethylene glycol 1500 cell hybridization protocol. Double-stained cells indicating fusion of BJA-B cells were detectable for up to 5 days. In parallel, L1236 cells were split into two fractions, stained with the fluorochromes, and mixed. No double-stained L1236 cells were detected. The same result was obtained when using FACS-sorted small mononuclear L1236 cells. It is thus concluded that the large multinucleated cells of the monoclonal H-RS cell line L1236 have emerged by endomitosis rather than by spontaneous cell fusion.
霍奇金淋巴瘤中多核细胞形成的机制尚未阐明。我们研究了H-RS细胞系L1236中的巨大多核细胞是否通过主要的较小细胞融合形成。作为融合试验的阳性对照，将B细胞淋巴瘤细胞系BJA-B来源的人B细胞分成两部分，分别用荧光染料CMTMR和CMFDA染色，并使用聚乙二醇1500细胞杂交方案进行融合。可检测到指示BJA-B细胞融合的双染细胞长达5天。同时，将L1236细胞分成两部分，用荧光染料染色并混合。未检测到双染的L1236细胞。使用流式细胞术分选的小单核L1236细胞时也得到了相同的结果。因此得出结论，单克隆H-RS细胞系L1236中的大多核细胞是通过核内有丝分裂而非自发细胞融合产生的。
