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黏液纤维肉瘤中多核化后伴巨细胞增殖的无丝分裂细胞分裂。

Multinucleation followed by an acytokinetic cell division in myxofibrosarcoma with giant cell proliferation.

作者信息

Ariizumi Takashi, Ogose Akira, Kawashima Hiroyuki, Hotta Tetsuo, Umezu Hajime, Endo Naoto

机构信息

Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.

出版信息

J Exp Clin Cancer Res. 2009 Mar 31;28(1):44. doi: 10.1186/1756-9966-28-44.

DOI:10.1186/1756-9966-28-44
PMID:19335880
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2669054/
Abstract

BACKGROUND

Multinucleated cells are frequently seen in association with a malignant neoplasm. Some of these multinucleated cells are considered to be neoplastic. The mechanism of neoplastic multinucleation remains unknown, but is considered to be induced by either cell-cell fusion or acytokinetic cell division. Myxofibrosarcoma consists of spindled and pleomorphic tumor cells and bizarre multinucleated giant cells. Some of these multinucleated cells are considered to be neoplastic.

METHODS

We studied the mitotic activity of the multinucleated cells by Ki-67 immunohistochemistry, and the dynamics and differentiation by live-cell video microscopy in the two myxofibrosarcoma cell lines to determine whether the mechanism of multinucleation is cell-cell fusion or acytokinetic cell division

RESULTS

A Ki-67 immunohistochemical analysis revealed a high positive rate of multinucleated cells, as well as mononuclear cells, and mitotic ability was shown in the multinucleated cells. In live-cell video microscopy, most of the multinucleated cells were induced via the process of acytokinetic cell division.

CONCLUSION

The current study indicates that a vulnerability of the cytoskeleton components, such as the contractile ring, causes multinucleation to occur from the telophase to the cytokinesis of the cell cycle.

摘要

背景

多核细胞常与恶性肿瘤相关。其中一些多核细胞被认为是肿瘤性的。肿瘤性多核化的机制尚不清楚，但被认为是由细胞 - 细胞融合或无胞质分裂的细胞分裂所诱导。黏液纤维肉瘤由梭形和多形性肿瘤细胞以及奇异的多核巨细胞组成。其中一些多核细胞被认为是肿瘤性的。

方法

我们通过Ki-67免疫组织化学研究了多核细胞的有丝分裂活性，并通过活细胞视频显微镜观察了两种黏液纤维肉瘤细胞系中的动态变化和分化情况，以确定多核化的机制是细胞 - 细胞融合还是无胞质分裂的细胞分裂。

结果

Ki-67免疫组织化学分析显示多核细胞以及单核细胞的阳性率较高，且多核细胞具有有丝分裂能力。在活细胞视频显微镜观察中，大多数多核细胞是通过无胞质分裂的细胞分裂过程诱导产生的。

结论

当前研究表明，细胞骨架成分（如收缩环）的脆弱性会导致在细胞周期的末期到胞质分裂阶段发生多核化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/8dab05e6bc30/1756-9966-28-44-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/a393b6e3f82e/1756-9966-28-44-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/e7d0e25d670f/1756-9966-28-44-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/a493040e4ed7/1756-9966-28-44-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/0b405ba08203/1756-9966-28-44-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/18d8d512aaee/1756-9966-28-44-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/8dab05e6bc30/1756-9966-28-44-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/a393b6e3f82e/1756-9966-28-44-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/e7d0e25d670f/1756-9966-28-44-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/a493040e4ed7/1756-9966-28-44-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/0b405ba08203/1756-9966-28-44-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/18d8d512aaee/1756-9966-28-44-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bf01/2669054/8dab05e6bc30/1756-9966-28-44-6.jpg

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