Hewett J, Nadeau V, Ferguson J, Moseley H, Ibbotson S, Allen J W, Sibbett W, Padgett M
School of Physics and Astronomy, University of St. Andrews, St. Andrews KY16 9SS, UK.
Photochem Photobiol. 2001 Mar;73(3):278-82.
A novel, compact and low-cost multispectral fluorescence imaging system with an integrated excitation light source is described. Data are presented demonstrating the application of this method to in vivo monitoring of fluorescence before, during and after topical 5-aminolevulinic acid photodynamic therapy of superficial skin cancers. The excitation source comprised a fluorescent tube with the phosphor selected to emit broadband violet light centered at 394 nm. The camera system simultaneously captured spectrally specific images of the fluorescence of the photosensitizer, protoporphyrin IX, the illumination profile and the skin autofluorescence. Real-time processing enabled images to be manipulated to create a composite image of high contrast. The application and validation of this method will allow further detailed studies of the characteristics and time-course of protoporphyrin IX fluorescence, during topical photodynamic therapy in human skin in vivo.
本文描述了一种新型、紧凑且低成本的多光谱荧光成像系统，该系统集成了激发光源。文中给出的数据展示了该方法在浅表皮肤癌局部5-氨基酮戊酸光动力治疗前、治疗期间和治疗后的荧光体内监测中的应用。激发源由一根荧光灯管组成，其荧光粉被选择用来发射以394nm为中心的宽带紫光。相机系统同时捕获了光敏剂原卟啉IX的荧光、照明轮廓和皮肤自发荧光的光谱特异性图像。实时处理使得图像能够被处理以创建高对比度的合成图像。该方法的应用和验证将有助于对人体皮肤局部光动力治疗过程中原卟啉IX荧光的特性和时间进程进行更详细的研究。
