Gothe U
Acta Histochem. 1979;64(1):71-7.
The iron binding reaction provides inconstant results with respect to the number of electron dense particles bound to cell surfaces if acetic acid has been used for the production of the colloid and for washing procedures in the course of iron binding. In the present paper it is shown that the dilution of colloidal iron hydroxide by adding acetic acid changes the size distribution of the colloidal particles, resulting in a marked decrease of iron binding. For that reason, a standard method is proposed in which hydrochloric acid is used instead of acetic acid. By this modification more reproducible results are gained with regard to the above mentioned quantitative aspects.
如果在铁结合过程中使用乙酸来制备胶体并用于洗涤步骤，那么铁结合反应在结合到细胞表面的电子致密颗粒数量方面会产生不稳定的结果。本文表明，通过添加乙酸来稀释氢氧化铁胶体，会改变胶体颗粒的大小分布，导致铁结合显著减少。因此，提出了一种标准方法，其中使用盐酸代替乙酸。通过这种改进，在上述定量方面获得了更可重复的结果。
