A colloidal iron silver impregnation method for acid mucopolysaccharide.

A simple and rapid method for the demonstration of acid mucopolysaccharide using colloidal iron, sulphosalicylic acid ammoniacal silver is described. Satisfactory staining of human as well as animal tissue was obtained following 10% neutral formol saline fixation and paraffin sections. There were no oxidation or hydrolysis sequences. The staining specificity was controlled using methylation and saponification. The distribution of the acid mucopolysaccharide demonstrated by this method was identical with that obtained by colloidal iron Perls' reaction.