Rossberg M, Theres K, Acarkan A, Herrero R, Schmitt T, Schumacher K, Schmitz G, Schmidt R
Max-Delbrück-Laboratorium in der Max-Planck-Gesellschaft, 50829 Cologne, Germany.
Plant Cell. 2001 Apr;13(4):979-88. doi: 10.1105/tpc.13.4.979.
A 57-kb region of tomato chromosome 7 harboring five different genes was compared with the sequence of the Arabidopsis genome to search for microsynteny between the genomes of these two species. For all five genes, homologous sequences could be identified in a 30-kb region located on Arabidopsis chromosome 1. Only two inversion events distinguish the arrangement of the five genes in tomato from that in Arabidopsis. Inversions were not detected when the arrangement of the five Arabidopsis genes was compared with the arrangement in the orthologous region of Capsella, a plant closely related to Arabidopsis. These results provide evidence for microcolinearity between closely and distantly related dicotyledonous species. The degree of microcolinearity found can be exploited to localize orthologous genes in Arabidopsis and tomato in an unambiguous way.
对番茄7号染色体上包含5个不同基因的57千碱基区域与拟南芥基因组序列进行比较,以寻找这两个物种基因组之间的微共线性。对于所有这5个基因,在拟南芥1号染色体上一个30千碱基的区域中可以鉴定出同源序列。只有两个倒位事件区分了番茄中这5个基因的排列与拟南芥中的排列。当将拟南芥这5个基因的排列与荠菜(一种与拟南芥密切相关的植物)直系同源区域的排列进行比较时,未检测到倒位。这些结果为近缘和远缘双子叶植物物种之间的微共线性提供了证据。所发现的微共线性程度可用于以明确的方式在拟南芥和番茄中定位直系同源基因。