Mao L, Begum D, Goff S A, Wing R A
Clemson University Genomics Institute, 100 Jordan Hall, Clemson, South Carolina 29634, USA.
Plant Physiol. 2001 Jul;126(3):1331-40. doi: 10.1104/pp.126.3.1331.
A 119-kb bacterial artificial chromosome from the JOINTLESS locus on the tomato (Lycopersicon esculentum) chromosome 11 contained 15 putative genes. Repetitive sequences in this region include one copia-like LTR retrotransposon, 13 simple sequence repeats, three copies of a novel type III foldback transposon, and four putative short DNA repeats. Database searches showed that the foldback transposon and the short DNA repeats seemed to be associated preferably with genes. The predicted tomato genes were compared with the complete Arabidopsis genome. Eleven out of 15 tomato open reading frames were found to be colinear with segments on five Arabidopsis bacterial artificial chromosome/P1-derived artificial chromosome clones. The synteny patterns, however, did not reveal duplicated segments in Arabidopsis, where over half of the genome is duplicated. Our analysis indicated that the microsynteny between the tomato and Arabidopsis genomes was still conserved at a very small scale but was complicated by the large number of gene families in the Arabidopsis genome.
来自番茄（Lycopersicon esculentum）11号染色体上无节位点的一个119 kb细菌人工染色体包含15个推定基因。该区域的重复序列包括一个类copia的LTR反转录转座子、13个简单序列重复、三个新型III型回文转座子拷贝以及四个推定的短DNA重复序列。数据库搜索表明，回文转座子和短DNA重复序列似乎更倾向于与基因相关联。将预测的番茄基因与完整的拟南芥基因组进行比较。发现15个番茄开放阅读框中的11个与五个拟南芥细菌人工染色体/P1衍生人工染色体克隆上的片段共线。然而，共线性模式并未揭示拟南芥中的重复片段，其基因组超过一半是重复的。我们的分析表明，番茄和拟南芥基因组之间的微共线性在非常小的尺度上仍然保守，但由于拟南芥基因组中大量的基因家族而变得复杂。
