3型一氧化氮合酶在反流性食管病变中的表达
Expression of nitric oxide synthase type 3 in reflux-induced esophageal lesions.
作者信息
Chandra R, Haines G K, Bentz B G, Shah P, Robinson A M, Radosevich J A
机构信息
Department of Otolaryngology--Head and Neck Surgery, Northwestern University School of Medicine, Chicago, Illinois, USA.
出版信息
Otolaryngol Head Neck Surg. 2001 Apr;124(4):442-7. doi: 10.1067/mhn.2001.114254.
BACKGROUND
The expression of endothelial constitutive nitric oxide synthase (NOS3) by squamous dysplasia and carcinomas of the head and neck has previously been described. We sought to compare NOS3 expression in squamous mucosa, glandular metaplasia, and adenocarcinoma of the esophagus.
METHODS
Forty paraffin-embedded specimens from 20 patients with adenocarcinoma were stained with anti-NOS3 monoclonal antibody. The percentage of cells stained and the intensity of staining were determined for squamous epithelium, metaplasia, and adenocarcinoma. Staining characteristics were statistically analyzed according to clinical variables.
RESULTS
NOS3 expression was significantly higher in adenocarcinoma and squamous epithelium compared with glandular metaplasia. Among the carcinomas, larger tumor size (T3/4), nodal positivity, and advanced TNM stage (III/IV) significantly correlated with increased NOS3 expression.
CONCLUSIONS
NOS3 is expressed in reflux-induced lesions of the esophagus. Glandular metaplasia shows basal levels of NOS3 that significantly increase with malignant transformation and tumor progression. The role of free radicals in carcinogenesis is being actively studied.
背景
先前已有关于头颈部鳞状发育异常及癌组织中内皮型一氧化氮合酶（NOS3）表达的描述。我们试图比较食管鳞状黏膜、腺化生及腺癌中NOS3的表达情况。
方法
用抗NOS3单克隆抗体对20例腺癌患者的40份石蜡包埋标本进行染色。测定鳞状上皮、化生及腺癌中染色细胞的百分比及染色强度。根据临床变量对染色特征进行统计学分析。
结果
与腺化生相比，腺癌和鳞状上皮中NOS3的表达明显更高。在癌组织中，更大的肿瘤大小（T3/4）、淋巴结阳性及晚期TNM分期（III/IV）与NOS3表达增加显著相关。
结论
NOS3在食管反流诱导的病变中表达。腺化生中NOS3呈基础水平表达，随着恶性转化和肿瘤进展显著增加。自由基在致癌过程中的作用正在积极研究中。
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