Holm S, Wahlin A
Acta Med Scand. 1979;205(7):587-91. doi: 10.1111/j.0954-6820.1979.tb06109.x.
Rabbits were immunized with human kidney homogenate. The rabbit sera, after absorption with human liver homogenate, showed antibody activity against human kidney, renal pelvis and urinary bladder. The sera were then absorbed with human renal pelvis and urinary bladder mucosa and subsequently showed no antibody activity against urinary bladder or renal pelvis. Immunofluorescence examinations showed fluorescing cells in kidney tubules, but not elsewhere. The finally absorbed antihuman kidney sera were used for indirect immunofluorescence examination of urinary sediments from patients with renal disease. Phase contrast microscopy was used simultaneously. Fluorescence was found in cells which in phase contrast microscopy were judged to be renal tubular cells. Fluorescing cells were often found in casts, but free cells were also seen. Immunofluorescence may thus provide a means of identifying renal tubular cells in urine.
用人类肾脏匀浆对兔子进行免疫。兔血清用人肝脏匀浆吸收后，显示出针对人类肾脏、肾盂和膀胱的抗体活性。然后用人类肾盂和膀胱黏膜对血清进行吸收，随后血清对膀胱或肾盂不再显示抗体活性。免疫荧光检查显示肾小管中有荧光细胞，但其他部位没有。最终吸收后的抗人肾血清用于对肾病患者尿液沉淀物进行间接免疫荧光检查。同时使用相差显微镜。在相差显微镜下判断为肾小管细胞的细胞中发现了荧光。在管型中经常发现荧光细胞，但也可见游离细胞。因此，免疫荧光可为识别尿液中的肾小管细胞提供一种方法。
