Pellet H, Minaire E, Cavailles C, Thonnerieux M
Bull Assoc Anat (Nancy). 1979 Sep;63(182):323-34.
Phase contrast microscopic observation of the urinary sediment has to be performed on freshly collected urine. This technique, which permits an excellent definition of the cells and the maintenance of intra cellular motility, gives therefore a great accuracy in differential diagnosis, especially for little round cells. Among these, polynuclear granulocytes are easily recognized, because of their lobulated nuclei and of their numerous intra cytoplasmic motile granulations. Lymphocytes and lymphoblasts have round, regular, homogeneous nuclei. Renal tubular cells exhibit eccentric nuclei, with smudged chromatine, and round, refringent cytoplasmic vacuoles. Rapid and easy, this technic should be used for routine microscopic study or urinary sediments.
尿沉渣的相差显微镜观察必须对新鲜采集的尿液进行。这项技术能够很好地界定细胞并维持细胞内的活动,因此在鉴别诊断中,尤其是对小圆细胞的鉴别诊断中具有很高的准确性。其中,多核粒细胞因其分叶状细胞核和众多胞质内活动颗粒而易于识别。淋巴细胞和淋巴母细胞有圆形、规则、均匀的细胞核。肾小管细胞呈现偏心核,染色质模糊,还有圆形、折光性的胞质空泡。这项技术快速简便,应用于尿沉渣的常规显微镜检查。