Bendriss-Vermare N, Barthélémy C, Durand I, Bruand C, Dezutter-Dambuyant C, Moulian N, Berrih-Aknin S, Caux C, Trinchieri G, Brière F
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
J Clin Invest. 2001 Apr;107(7):835-44. doi: 10.1172/JCI11734.
Three distinct dendritic cell (DC) subsets capable of stimulating allogeneic naive T cells were isolated from human thymus. The most abundant subset was represented by plasmacytoid DCs (pDCs), which secreted high amounts of IFN-alpha upon stimulation with inactivated influenza virus and thus likely correspond to the recently identified peripheral blood natural IFN-alpha/beta-producing cells (IPCs). Like those latter cells, thymic pDCs had distinctive phenotypic features (i.e., Lin(-), HLA-DR(int), IL-3R alpha(hi), CD45RA(hi), CD11c(-), CD13(-), and CD33(lo)) and developed into mature DCs upon culture in IL-3 and CD40L. Of the two other DC subsets, one displayed a phenotype of immature myeloid DCs (imDCs) (HLA-DR(int), CD11c(+), CD13(+), CD33(+)), and the other represented HLA-DR(hi) CD11c(+) mature DCs (mDCs). Since they also expressed DC-LAMP, these mDCs appear to correspond to interdigitating dendritic cells (IDCs). Thymic pDCs, but not myeloid imDCs, strongly expressed lymphoid-specific transcripts such as pre-T alpha, lambda-like, and Spi-B, thereby suggesting a possible lymphoid origin. The detection of Spi-B mRNA, not only upon in vitro maturation of pDCs, but also in freshly purified IDCs, suggests that in vivo pDCs may differentiate into IDCs.
从人胸腺中分离出了三种能够刺激同种异体初始T细胞的不同树突状细胞（DC）亚群。数量最多的亚群是浆细胞样DC（pDC），在用灭活流感病毒刺激后，它们会分泌大量的IFN-α，因此可能与最近鉴定出的外周血天然IFN-α/β产生细胞（IPC）相对应。与后一种细胞一样，胸腺pDC具有独特的表型特征（即Lin(-)、HLA-DR(int)、IL-3Rα(hi)、CD45RA(hi)、CD11c(-)、CD13(-)和CD33(lo)），并在IL-3和CD40L中培养后发育为成熟DC。在另外两个DC亚群中，一个表现出未成熟髓样DC（imDC）的表型（HLA-DR(int)、CD11c(+)、CD13(+)、CD33(+)），另一个代表HLA-DR(hi) CD11c(+)成熟DC（mDC）。由于这些mDC也表达DC-LAMP，它们似乎对应于交错突细胞（IDC）。胸腺pDC而非髓样imDC强烈表达淋巴样特异性转录本，如前Tα、λ样和Spi-B，从而提示可能的淋巴样起源。不仅在pDC体外成熟时，而且在新鲜纯化的IDC中都检测到Spi-B mRNA，这表明体内pDC可能分化为IDC。
