Cella M, Facchetti F, Lanzavecchia A, Colonna M
Basel Institute for Immunology, CH-4005 Basel, Switzerland.
Nat Immunol. 2000 Oct;1(4):305-10. doi: 10.1038/79747.
Plasmacytoid dendritic cells (PDCs) are a subset of dendritic cells present in human blood and inflamed lymph nodes. Here we show that blood PDCs, when stimulated with influenza virus and CD40L in vitro, undergo a maturation process characterized by up-regulation of major histocompatibility complex proteins and adhesion and costimulatory molecules. In addition, PDCs down-regulate CXCR3 and L-selectin, which mediate migration and homing of these cells into the lymph node. Mature PDCs efficiently stimulate T cells and drive a potent TH1 polarization in vitro, which is mediated by the synergistic effect of interleukin 12 and type 1 interferon. In vivo, mature PDCs are found in secondary lymphoid organs, where they represent the principal source of type 1 interferon during inflammation. Thus, PDCs probably participate in antiviral and pro-inflammatory responses, rather than in TH2 polarization and tolerance induction.
浆细胞样树突状细胞（pDC）是存在于人体血液和炎症淋巴结中的树突状细胞亚群。我们在此表明，血液中的pDC在体外受到流感病毒和CD40L刺激时，会经历一个成熟过程，其特征是主要组织相容性复合体蛋白以及黏附分子和共刺激分子上调。此外，pDC下调CXCR3和L-选择素，这两种分子介导这些细胞迁移至淋巴结并归巢。成熟的pDC在体外能有效刺激T细胞并驱动强烈的TH1极化，这是由白细胞介素12和1型干扰素的协同作用介导的。在体内，成熟的pDC存在于二级淋巴器官中，在炎症期间它们是1型干扰素的主要来源。因此，pDC可能参与抗病毒和促炎反应，而非参与TH2极化和耐受性诱导。
