Kwag O G, Kim S O, Choi J H, Rhee I K, Choi M S, Rhee S J
Department of Nursing Science, Taegu Science College, Taegu, Korea 702-722.
J Nutr. 2001 Apr;131(4):1297-301. doi: 10.1093/jn/131.4.1297.
The purpose of the present study was to investigate the effects of vitamin E on microsomal phospholipase A2 activity and the arachidonic acid cascade in the kidneys of streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley male rats weighing 100 +/- 10 g were randomly assigned to one normal and three STZ-induced diabetic groups. The diabetic groups were fed a vitamin E-free diet (the DM-0E group), 40 mg vitamin E/kg diet (the DM-40E group) or a 400 mg vitamin E/kg diet (the DM-400E group). The kidney vitamin E concentrations were 59 and 49% lower in the DM-0E and DM-40E groups, respectively, than in the normal group. The kidney thiobarbituric acid reactive substance concentrations in the DM-0E, DM-40E and DM-400E groups were 119, 84 and 33% greater, respectively, than that in the normal group. The concentration in the DM-400E group was 39% lower than that in the DM-0E group. The phospholipase A2 (PLA2) activity in the kidney microsomes of the DM-0E-40E and DM-400E groups were 88, 58 and 35% greater, respectively, than that in the normal group. The activity in the DM-400E group was 28% lower than that in the DM-0E group and 16% lower than that in the DM-40E group. The differences in the phospholipids in the kidney microsomes included reductions in the phosphatidylcholine and phosphatidylethanolamine compositions. Phosphatidylethanolamine hydrolysis in the kidney microsomes of the DM-0E and DM-40E groups were 84 and 64%, which did not differ from the DM-400E group. The formation of thromboxane A2 (TXA2) in the kidney microsomes was 137 and 70% greater in the DM-0E and DM-40E groups, respectively, than in the normal group. TXA2 formation did not differ between the DM-400E and normal groups. The formation of prostacyclin in the kidney microsomes was 60 and 44% lower in the DM-0E and DM-40E groups, respectively, than in the normal group, whereas the DM-400E group did not differ from that in the normal group. The ratio of prostacyclin to TXA2 was 82 and 65% lower than normal in the DM-0E and DM-40E groups, respectively. Kidney function appears to be improved by vitamin E supplementation due to its antithrombus action, which in turn controls the arachidonic acid cascade system.
本研究的目的是探讨维生素E对链脲佐菌素（STZ）诱导的糖尿病大鼠肾脏微粒体磷脂酶A2活性及花生四烯酸级联反应的影响。将体重100±10 g的Sprague-Dawley雄性大鼠随机分为1个正常组和3个STZ诱导的糖尿病组。糖尿病组分别给予不含维生素E的饮食（DM-0E组）、40 mg维生素E/kg饮食（DM-40E组）或400 mg维生素E/kg饮食（DM-400E组）。DM-0E组和DM-40E组肾脏维生素E浓度分别比正常组低59%和49%。DM-0E组、DM-40E组和DM-400E组肾脏硫代巴比妥酸反应性物质浓度分别比正常组高119%、84%和33%。DM-400E组浓度比DM-0E组低39%。DM-0E组、DM-40E组和DM-400E组肾脏微粒体中磷脂酶A2（PLA2）活性分别比正常组高88%、58%和35%。DM-400E组活性比DM-0E组低28%，比DM-40E组低16%。肾脏微粒体中磷脂的差异包括磷脂酰胆碱和磷脂酰乙醇胺组成的减少。DM-0E组和DM-40E组肾脏微粒体中磷脂酰乙醇胺的水解率分别为84%和64%，与DM-400E组无差异。DM-0E组和DM-40E组肾脏微粒体中血栓素A2（TXA2）的生成分别比正常组高137%和70%。DM-400E组与正常组之间TXA2生成无差异。DM-0E组和DM-40E组肾脏微粒体中前列环素的生成分别比正常组低60%和44%，而DM-400E组与正常组无差异。DM-0E组和DM-40E组前列环素与TXA2的比值分别比正常组低82%和65%。补充维生素E似乎可改善肾功能，这归因于其抗血栓作用，进而控制花生四烯酸级联系统。
