Xu L H, Jiao X Y, Ji Z L
Department of Plastic Surgery, Changhai Hospital, Second Military Medical University, Shanghai, P. R. China 200433.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Mar;15(2):118-21.
To investigate the skin regeneration using cultured human keratinocytes with collagen sponge transplanted into thickness wound of nude mice.
Human foreskin from foreskin ectomy procedures was detached with 0.5% Dispase II. Epidermis sheets were separated from dermis and digested with 0.05% Trypsin into single cell suspension. Keratinocytes were cultured and seeded into collagen sponge during logarithmic growth phase. After 3 days, the keratinocytes-collagen sponge were grafted on full thickness wound of nude mice, compared with simple collagen sponge without keratinocytes. The histological, immunohistochemical examination and electron microscopy were detected.
After the epidermal substitute was grafted onto wound, the human keratinocytes were able to further proliferate and differentiate and develop into new epithelia. Compared with the control group, the wound healed earlier and contracted less, epithelia matured earlier, and the collagen fiber was less beneath epithelia.
Keratinocytes can grow on collagen sponge and migrate onto wound to develop into stratified epithelia and inhibit wound contract. The keratinocyte graft can be used to repair skin defect.
研究将培养的人角质形成细胞与胶原海绵移植到裸鼠全层伤口后皮肤的再生情况。
从包皮环切手术获取的人包皮用0.5%的Dispase II分离。表皮片与真皮分离，并用0.05%的胰蛋白酶消化成单细胞悬液。角质形成细胞进行培养，并在对数生长期接种到胶原海绵上。3天后，将角质形成细胞-胶原海绵移植到裸鼠全层伤口上，与不含角质形成细胞的单纯胶原海绵作比较。进行组织学、免疫组织化学检查及电子显微镜观察。
将表皮替代物移植到伤口后，人角质形成细胞能够进一步增殖、分化并发育成新的上皮组织。与对照组相比，伤口愈合更早，收缩更小，上皮组织成熟更早，上皮组织下的胶原纤维更少。
角质形成细胞能够在胶原海绵上生长并迁移到伤口，发育成复层上皮组织并抑制伤口收缩。角质形成细胞移植物可用于修复皮肤缺损。
