Deng S L, Yuan T, Liao W H
Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, P. R. China 400042.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Mar;15(2):74-6.
To observe the effect of nerve growth factor (NGF) and nimodipine (NP) on fetal spinal cord graft in repair of injury of spinal cord.
A total of 144 adult Wistar rats were included in this study. All were made as the hemi-section cavity injury model at the lumbar enlargement and divided into three groups: fetal spinal cord graft (group Tr), fetal spinal cord graft with NGF (group TN), and fetal spinal cord graft with NGF and NP (group TNN). The intracellular concentration of free ionic calcium was measured at the 4th, 8th, and 24th hour, and superoxidase (SOD) and malondialdehyde (MDA) at 3rd, 6th, 12th, 24th and 72nd hour after operation.
After spinal cord was injured, the concentration of MDA and intracellular concentration of free ionic calcium increased and reached to the peak at the 6th and 8th hour respectively, but SOD decreased and at 24th hour to its vale. The MDA was significantly lower in group TN than in group Tr, while the SOD was higher (P < 0.05). There was no significant difference on intracellular free ionic calcium concentration between group Tr and TN. The concentration of SOD of group TNN was the highest and the intracellular concentration of free ionic calcium was the lowest in the three groups (P < 0.05). The weekly mortality was 33%, 31%, 17% respectively in group Tr, TN and TNN. The mortality of group TNN was significantly lower than the other two groups (P < 0.01).
Although the fetal spinal cord graft is an effective method to repair laboratory spinal cord injury, NGF and ND can interrupt secondary injury and increase survival rate of the host.
观察神经生长因子（NGF）和尼莫地平（NP）对胎儿脊髓移植修复脊髓损伤的作用。
本研究共纳入144只成年Wistar大鼠。全部大鼠在腰膨大处制作半横断空洞损伤模型，并分为三组：胎儿脊髓移植组（Tr组）、胎儿脊髓移植联合NGF组（TN组）和胎儿脊髓移植联合NGF及NP组（TNN组）。分别于术后第4、8和24小时测定细胞内游离钙离子浓度，于术后第3、6、12、24和72小时测定超氧化物歧化酶（SOD）和丙二醛（MDA）。
脊髓损伤后，MDA浓度和细胞内游离钙离子浓度升高，分别在第6和8小时达到峰值，而SOD下降，并在第24小时降至最低值。TN组MDA显著低于Tr组，而SOD高于Tr组（P<0.05）。Tr组和TN组细胞内游离钙离子浓度差异无统计学意义。TNN组SOD浓度在三组中最高，细胞内游离钙离子浓度在三组中最低（P<0.05）。Tr组、TN组和TNN组每周死亡率分别为33%、31%、17%。TNN组死亡率显著低于其他两组（P<0.01）。
尽管胎儿脊髓移植是修复实验性脊髓损伤的有效方法，但NGF和NP可阻断继发性损伤并提高宿主存活率。
