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[鸡同种异体带鞘肌腱移植的实验研究]

[Experimental study of allogenic tendon with sheath grafting in chicken].

作者信息

Zhang Y L, Wang S L, Gao X S

机构信息

Department of Hand Surgery, Beijing Ji Shui Tan Hospital, Beijing, P. R. China 100035.

出版信息

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2001 Mar;15(2):92-5.

Abstract

OBJECTIVE

To investigate availability of deep freeze stored allogenic tendon with sheath grafting in repairing the tendon and sheath defect in the II area of flexor digitorum tendon.

METHODS

Sixty chickens with tendon and sheath defect were divided into 2 groups randomly, group A was treated with allogenic grafting and group B was treated with autogenic grafting, these two groups were divided into two subgroups respectively, they were, group A1 allogenic tendon with whole sheath grafting, group A2 allogenic tendon with partial sheath grafting, group B1 autogenic tendon with whole sheath grafting and group B2 autogenic tendon with whole sheath grafting. All the allogenic grafts were treated by deep freeze. Histomorphological study, histoimmunological study and slipping function of the grafts were measured after operation.

RESULTS

In group A1 and B1, the local reaction was sever, the nutrition of tendon graft was barricaded by the whole sheath resulting in adhesion, degeneration and necrosis. In group A2 and B2, the tendon graft healed well and little adhesion existed between tendon and sheath. The results showed that there were significant differences between tendon grafting with whole sheath and tendon grafting with partial sheath.

CONCLUSION

Deep freeze store can reduce the immunogenicity of allogenic tendon with sheath. Allogenic tendon with partial sheath grafting can be used as a new biological material for repairing the tendon and sheath defect.

摘要

目的

探讨深低温保存的同种异体带腱鞘肌腱移植修复指屈肌腱Ⅱ区肌腱及腱鞘缺损的可行性。

方法

将60只造成肌腱和腱鞘缺损的鸡随机分为2组,A组采用同种异体移植,B组采用自体移植,两组再各自分为两个亚组,即A1组同种异体全腱鞘肌腱移植、A2组同种异体部分腱鞘肌腱移植、B1组自体全腱鞘肌腱移植和B2组自体全腱鞘肌腱移植。所有同种异体移植物均经深低温处理。术后对移植物进行组织形态学研究、组织免疫学研究及滑动功能测定。

结果

A1组和B1组局部反应严重,全腱鞘阻碍了肌腱移植物的营养供应,导致粘连、变性和坏死。A2组和B2组肌腱移植物愈合良好,肌腱与腱鞘间粘连少。结果表明,全腱鞘肌腱移植与部分腱鞘肌腱移植之间存在显著差异。

结论

深低温保存可降低同种异体带腱鞘肌腱的免疫原性。同种异体部分腱鞘肌腱移植可作为修复肌腱及腱鞘缺损的一种新型生物材料。

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