Carter N S, Landfear S M, Ullman B
Dept of Biochemistry and Molecular Biology, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, Portland, OR 97201-3098, USA.
Trends Parasitol. 2001 Mar;17(3):142-5. doi: 10.1016/s1471-4922(00)01806-7.
Protozoan parasites are incapable of synthesizing purine nucleotides de novo and so must salvage preformed purines from their hosts. This process of purine acquisition is initiated by the translocation of preformed host purines across parasite or host membranes. Here, we report upon the identification and isolation of DNAs encoding parasite nucleoside transporters and the functional characterization of these proteins in various expression systems. These potential approaches provide a powerful approach for a thorough molecular and biochemical dissection of nucleoside transport in protozoan parasites.
原生动物寄生虫无法从头合成嘌呤核苷酸，因此必须从宿主那里挽救预先形成的嘌呤。嘌呤获取的这个过程是由预先形成的宿主嘌呤跨寄生虫或宿主膜的转运引发的。在这里，我们报告了编码寄生虫核苷转运蛋白的DNA的鉴定和分离，以及这些蛋白在各种表达系统中的功能特性。这些潜在的方法为全面分子和生化剖析原生动物寄生虫中的核苷转运提供了一种强有力的方法。
