Zini A, Bielecki R, Phang D, Zenzes M T
Division of Urology, Department of Surgery, University of Toronto, Ontario, Toronto, Canada.
Fertil Steril. 2001 Apr;75(4):674-7. doi: 10.1016/s0015-0282(00)01796-9.
To evaluate two different assays of human sperm DNA integrity, DNA denaturation (DD) and DNA fragmentation (DF), and to correlate these with standard semen parameters.
Prospective, observational study.
University infertility clinic.
PATIENT(S): Forty consecutive semen samples from 33 nonazoospermic men presenting for infertility evaluation and 7 fertile men presenting for vasectomy.
INTERVENTION(S): Assessment of sperm concentration, motility, morphology, DD and DF.
MAIN OUTCOME MEASURE(S): Sperm DD and DF in fertile and infertile men.
RESULT(S): The mean (+/-SE) rates of DD and DF were significantly higher in infertile subjects compared to fertile controls, respectively: 25.4 +/- 3.0 vs. 10.2 +/- 2.3 (P=.028) and 27.6 +/- 2.5 vs. 13.3 +/- 2.5% (P=.016). DF and DD correlated strongly (r = 0.71, P<.0001). Also, DD and DF correlated negatively with standard semen parameters (concentration, motility, and morphology), the strongest correlation being with sperm motility.
CONCLUSION(S): The strong correlation between sperm DD and DF, and the higher levels of sperm DNA damage in infertile compared with fertile men, indicate that male infertility is associated with poor sperm DNA integrity. Although infertile men may father children with assisted conception, fertilization with DNA-damaged spermatozoa may increase the risk of genetic disease in the offspring.
评估两种不同的人类精子DNA完整性检测方法，即DNA变性（DD）和DNA片段化（DF），并将其与标准精液参数相关联。
前瞻性观察性研究。
大学不育诊所。
33名因不育前来评估的非无精子症男性和7名因输精管切除术前来的生育男性连续提供的40份精液样本。
评估精子浓度、活力、形态、DD和DF。
生育男性和不育男性的精子DD和DF。
与生育对照组相比，不育受试者的DD和DF平均（±SE）率显著更高，分别为：25.4±3.0对10.2±2.3（P = 0.028）和27.6±2.5对13.3±2.5%（P = 0.016）。DF和DD相关性很强（r = 0.71，P < 0.0001）。此外，DD和DF与标准精液参数（浓度、活力和形态）呈负相关，最强的相关性是与精子活力。
精子DD和DF之间的强相关性，以及不育男性与生育男性相比更高水平的精子DNA损伤，表明男性不育与精子DNA完整性差有关。虽然不育男性可能通过辅助受孕生育子女，但用DNA受损的精子受精可能会增加后代患遗传病的风险。
