Zini Armand, Fischer Marc Anthony, Sharir Sharon, Shayegan Bobby, Phang Donna, Jarvi Keith
Division of Urology, Department of Surgery, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada.
Urology. 2002 Dec;60(6):1069-72. doi: 10.1016/s0090-4295(02)01975-1.
To examine the prevalence of abnormal sperm DNA denaturation (DD), a marker of sperm DNA integrity, in a group of fertile and infertile men.
Eighty-eight nonazoospermic, infertile men and 13 fertile men underwent standard semen analysis and acridine orange sperm DNA integrity studies. Standard semen parameters (sperm concentration, motility, and morphology) and sperm DNA integrity (expressed as the percentage of spermatozoa with DD) were measured.
Of the 88 infertile men, 13 had completely normal semen parameters and the remaining 75 had at least one abnormal semen parameter. The mean (+/-SE) sperm DD level was significantly lower in the population of infertile men with normal semen parameters compared with those having abnormal parameters (11.1% +/- 3.7% versus 23.1% +/- 1.8%, respectively, P <0.001). Only 1 (8%) of the 13 men with normal semen parameters had elevated sperm DD (greater than 30%, verified on two separate analyses) compared with 13 (17%) of the 75 infertile men with abnormal semen parameters (P >0.05). None of the fertile controls had elevated sperm DD. We observed significant inverse correlations between the sperm DD and sperm motility, morphology, and concentration (P <0.001).
Our data show that sperm DD negatively correlates with standard semen parameters and that an isolated abnormality of sperm DD, a marker of sperm DNA integrity, is uncommon in infertile men. Additional studies are needed to support the notion that isolated abnormalities of sperm DNA integrity may represent a new diagnosis for men with unexplained infertility.
在一组有生育能力和不育的男性中，检测精子DNA变性（DD）这一精子DNA完整性标志物的异常发生率。
88名非无精子症的不育男性和13名有生育能力的男性接受了标准精液分析和吖啶橙精子DNA完整性研究。测量了标准精液参数（精子浓度、活力和形态）以及精子DNA完整性（以发生DD的精子百分比表示）。
88名不育男性中，13名精液参数完全正常，其余75名至少有一项精液参数异常。精液参数正常的不育男性群体的平均（±标准误）精子DD水平显著低于精液参数异常的男性群体（分别为11.1%±3.7%和23.1%±1.8%，P<0.001）。精液参数正常的13名男性中只有1名（8%）精子DD升高（大于30%，经两次独立分析证实），而精液参数异常的75名不育男性中有13名（17%）精子DD升高（P>0.05）。所有有生育能力的对照者精子DD均未升高。我们观察到精子DD与精子活力、形态和浓度之间存在显著的负相关（P<0.001）。
我们的数据表明，精子DD与标准精液参数呈负相关，而且作为精子DNA完整性标志物的单纯精子DD异常在不育男性中并不常见。需要进一步研究来支持单纯精子DNA完整性异常可能代表不明原因不育男性的一种新诊断这一观点。
